▶ Specifications
| Scale |
8-channel scale |
mini scale |
midi scale |
maxi scale |
| Starting culture volume |
1 - 1.5 ml |
1 - 10 ml |
20 - 50 ml |
100 - 200 ml |
| Preparation time |
< 5 min |
< 5 min |
< 10 min |
< 15 min |
| Elution volume |
100 μl |
100 μl |
500 μl |
1 ml |
| Expected DNA yield |
Up to 10 μg |
Up to 20 μg |
Up to 200 μg |
Up to 500 μg |
| Expected DNA purity |
A260/280 > 1.8 |
* Differences in the quantified value may occur depending on the types of plasmids.
▶ Components
| Components |
K-3601*
(100 reaction) |
K-3600**
(500 reaction) |
| PM1 Buffer (Resuspension) |
25 ml x 1 ea |
110 ml x 1 ea |
| P2 Buffer (Lysis) |
25 ml x 1 ea |
110 ml x 1 ea |
| PC Buffer (Neutralization) |
25 ml x 1 ea |
110 ml x 1 ea |
| PB Buffer (Binding) |
75 ml x 1 ea |
180 ml x 2 ea |
| WE Buffer (Washing) |
120 ml x 1 ea |
240 ml x 2 ea |
| EA Buffer (Elution) |
15 ml x 1 ea |
60 ml x 1 ea |
| RNase A powder, lyophilized |
3 mg x 1 ea |
12 mg x 1 ea |
| Magnetic nano bead |
9 ml x 1 ea |
40 ml x 1 ea |
| User's Guide |
1 ea |
1 ea |
*mini – 100 rxn, midi – 10 rxn, maxi – 4 rxn
**mini – 500 rxn, midi – 50 rxn, maxi – 18 rxn
▶ Experimental data
 |
M1; Bioneer 100 bp ladder
1; 2 kb plasmid DNA purified with Bioneer MagListo™ 5M Plasmid Extraction Kit
2; 2 kb plasmid DNA purified with competitor P kit
3; 2 kb plasmid DNA purified with competitor I kit
M2; Bioneer 1 kb ladder |
Figure 1. Compared purified plasmid DNA yields between MagListo™ 5M Plasmid Extraction Kit and competitors' products (magnetic bead Type).
| Sample ID |
Nucleic Acid Conc. |
260/280 |
260/230 |
| Bioneer |
117.9 ng/µl |
2.03 |
1.91 |
| Competitor P |
95.9 ng/µl |
2.05 |
1.91 |
| Competitor I |
90.9 ng/µl |
2.03 |
1.9 |
Figure 2. Electrophoresis data of three scale plasmid DNA extraction protocols (mini, midi, maxi) treated with endonuclease restriction (500 ng quantified).
M1; Bioneer 100 bp ladder
1; Plasmid DNA purified with mini scale protocol (1)
2; Plasmid DNA purified with mini scale protocol/digested by EcoRI (1)
3; Plasmid DNA purified with mini scale protocol (2)
4; Plasmid DNA purified with mini scale protocol/digested by EcoRI (2)
5; Plasmid DNA purified with midi scale protocol (1)
6; Plasmid DNA purified with midi scale protocol/digested by EcoRI (1)
7; Plasmid DNA purified with midi scale protocol (2)
8; Plasmid DNA purified with midi scale protocol/digested by EcoRI (2)
9; Plasmid DNA purified with maxi scale protocol
10; Plasmid DNA purified with miaxi scale protocol/digested by EcoRI
M2; Bioneer 1 kb ladder
Figure 3. Electrophoresis data of 500 ng plasmids of various sizes purified with MagListo™ 5M Plasmid Extraction Kit.
M1; Bioneer 100 bp ladder
1; 3.5 kb plasmid DNA
2; 5.4 kb plasmid DNA
3; 8 kb plasmid DNA
M2; Bioneer 1 kb ladder
Figure 4. High quality sequencing data of pGEM-B1 clones purified with MagListo™ 5M Plasmid Extraction Kit.
