AccuPrep® Blood RNA Extraction Kit (50 reactions)

AccuPrep® Blood RNA Extraction Kit is a product capable of extracting high-purity RNA from whole blood. The isolated RNA can be used for various experiments such as RT-PCR, quantitative real time RT-PCR, and microarray analysis. Using AccuPrep® Blood RNA Extraction Kit, you can extract high-yield RNA without degradation.

Spin column type

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Cat. No.

K-3144G

Product Overview

Features and Benefits

High-quality, high-yield
High-quality, high-yield extraction from blood.
High binding capacity
Up to 120 µg using silica based RNA binding columns
Minimized contamination
Columns individually sterilized and packaged
Convenience
Etanol already added to the Washing buffer

Application

• cDNA synthesis

• RT-PCR

• Quantitative real-time PCR

• Northern blot analysis

• RNA sequencing

Technical/Specs

▶ Specifications

Sample	Amount	RNA Yield	Purity*
Human Blood	200 μl	2~10 μg	A_260/280 > 2.0 A_260/230 > 1.7
Mouse Blood		5~10 μg
Pig Blood		≥ 2 μg

*Depending on the type of sample, there may be differences in the measured values.

▶ Components

Components	K-3144 (50 reaction)
RDD Buffer (RNA Binding)	15 ml X 1 ea
RV Buffer (RNA Recovery)	15 ml X 1 ea
RWA1 Buffer (1^st Washing)	40 ml X 1 ea
RWA2 Buffer (2^nd Washing)	70 ml X 1 ea
ER Buffer (Elution)	10 ml X 1 ea
Proteinase K, lyophilized	25 mg X 1 ea
User's Guide	1 ea

▶ Experimental data

Prep kit	Conc. (ng/μl)	260/280	260/230
Bioneer	565.061	2.163	2.215
Bioneer	344.395	2.135	2.248
Company Q	19.533	1.707	0.195
Company Q	24.621	1.892	1.938

Figure 1. Blood RNA extraction using AccuPrep^® Blood RNA Extraction Kit
RNA extraction from Mouse whole blood. DNase was not treated.

Prep kit	Conc. (ng/μl)	260/280	260/230
Bioneer	97.415	2.123	2.088
Bioneer	89.989	2.078	1.548
Company Q	25.396	1.772	0.897
Company Q	23.745	1.731	0.773

Figure 2. Blood RNA extraction using AccuPrep^® Blood RNA Extraction Kit
RNA extraction from Pig whole blood. DNase was not treated.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

▶ Ordering Information

Cat. No.	Product Description	Option
K-3144	AccuPrep^® Blood RNA Extraction Kit	50 reactions