▶ Specifications
| Sample |
Amount |
DNA Yield |
| Stool |
100 mg |
2 - 5 μg |
* Depending on the type of sample, there may be differences in the measured values.
▶ Components
| Components |
K-3036 |
| SL Buffer (Stool Lysis) |
50 ml x 1ea |
| SB Buffer (Binding) |
50 ml x 1ea |
| WA1 Buffer (1st Washing) |
40 ml x 1ea |
| W2 Buffer (2nd Washing) |
80 ml x 1ea |
| EA Buffer (Elution) |
25 ml x 1ea |
| Proteinase K, lyophilized |
25 mg x 2 ea |
| AccuPrep® Binding Column-I |
100 ea |
| Collection Tube (for filtration) |
100 ea |
| 1.5 ml tubes (for elution) |
100 ea |
| Quick Manual |
1 ea |
▶ Workflow
▶ Experimental data
Figure 1. Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit
- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (D-1070, Bioneer)
- Lane M2; Molecular weight marker, 1 kb ladder (D-1040, Bioneer)
- Lane 1~3; DNA purified with Bioneer's AccuPrep® Stool DNA Extraction Kit.
100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)
Figure 2. PCR results of H. pylori DNA extracted with AccuPrep® Stool DNA Extraction Kit
- Lane M; Molecular weight marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~4; PCR results for H. pylori detection
