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AccuPrep® Stool DNA Extraction Kit (100 reactions)
AccuPrep® Stool DNA Extraction Kit is a product that can be extracted high purity genomic DNA removing various PCR inhibitors from fresh or frozen stools of various animals including humans
Spin column type
Features and Benefits
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High purity genomic DNA extraction from human and various animal stools
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Using silica based DNA binding column with high binding capacity up to 30 µg
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Genomic DNA extraction with various PCR inhibitors (heparin, bilirubin, etc.) completely removed using Stool Lysis buffer (SL Buffer) optimized for various stool samples
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Easy to use because ethanol is added to Washing buffer
Application
• PCR
• Real-time PCR
• SNP genotyping
• Pathogen characterization
▶ Specifications
Sample | Amount | DNA Yield |
Stool | 100 mg | 2 - 5 μg |
* Depending on the type of sample, there may be differences in the measured values.
▶ Components
Components | K-3036 |
SL Buffer (Stool Lysis) | 50 ml x 1ea |
SB Buffer (Binding) | 50 ml x 1ea |
WA1 Buffer (1st Washing) | 40 ml x 1ea |
W2 Buffer (2nd Washing) | 80 ml x 1ea |
EA Buffer (Elution) | 25 ml x 1ea |
Proteinase K, lyophilized | 25 mg x 2 ea |
AccuPrep® Binding Column-I | 100 ea |
Collection Tube (for filtration) | 100 ea |
1.5 ml tubes (for elution) | 100 ea |
Quick Manual | 1 ea |
▶ Workflow
▶ Experimental data
Figure 1. Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit
- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (D-1070, Bioneer)
- Lane M2; Molecular weight marker, 1 kb ladder (D-1040, Bioneer)
- Lane 1~3; DNA purified with Bioneer's AccuPrep® Stool DNA Extraction Kit.
100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)
Figure 2. PCR results of H. pylori DNA extracted with AccuPrep® Stool DNA Extraction Kit
- Lane M; Molecular weight marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~4; PCR results for H. pylori detection
◈ Manual
• One Page Protocol: AccuPrep® Stool DNA Extraction Kit
• User Guide: AccuPrep® Stool DNA Extraction Kit
◈ MSDS
• MSDS_AccuPrep® Stool DNA Extraction Kit_SL Buffer
• MSDS_AccuPrep® Stool DNA Extraction Kit_SB Buffer
• MSDS_AccuPrep® Stool DNA Extraction Kit_WA1 Buffer
• MSDS_AccuPrep® Stool DNA Extraction Kit_W2 Buffer
• MSDS_AccuPrep® Stool DNA Extraction Kit_EA Buffer
• MSDS_AccuPrep® Stool DNA Extraction Kit_Proteinase K
◈ Brochure
• AccuPrep® nucleic acid extraction kit series
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
Cat. No. | Product Description | Option | Price |
K-3036 | AccuPrep® Stool DNA Extraction Kit | 100 reactions | Inquire |
KB-0111 | Proteinase K Powder | 25 mg X 2 | $KB-0111 |