AccuPower® Multiplex PCR PreMix allows to generate 20 multiplexed amplification products in a single tube. By applying antibody-based HotStart Top DNA Polymerase, it reduces non-specific reactions such as mis-priming and primer-dimer during DNA polymerase reaction at a low temperature. This product is applicable for normal and genotyping assays using multiplex PCR technology, along with semi-quantitative gene expression experiments using cDNA.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※MasterMix product is shipped in dry ice.
Generation of 20 multiplexed amplification products in a single tube.
Minimized unnecessary reactions such as mis-priming and primer dimer with excellent specificity and amplification efficiency than with other products when compared with same PCR cycles by the use of Hotstart Top DNA polymerase
PCR condition optimization not required with precise specificity of the product only requiring addition of template DNA and primers for anybody to simultaneously amplify the target DNA
Enhanced stability compared to solution-type products allowing delivery at room temperature by addition of stabilizing agents
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables through the mass production under ISO 9001 quality system
• STR analysis
• Molecular diagnostic analysis
• Qualitative, semi-qualitative gene expression assay
• Mutant screening
• Transgenic organism analysis
• Genotyping assay
Figure. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.
10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl2).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 65°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer)
