AccuPower® Multiplex PCR PreMix & Master Mix

AccuPower® Multiplex PCR PreMix allows to generate 20 multiplexed amplification products in a single tube. By applying antibody-based HotStart Top DNA Polymerase, it reduces non-specific reactions such as mis-priming and primer-dimer during DNA polymerase reaction at a low temperature. This product is applicable for normal and genotyping assays using multiplex PCR technology, along with semi-quantitative gene expression experiments using cDNA.

※Master Mix product is shipped in dry ice.

Application Note

• Application Note_Multiplex PCR PreMix PCR_Protozoa Penal

As low as $84.00
Cat. No.

Features and Benefits

  • Multiplex PCR

    Generation of 20 multiplexed amplification products in a single tube.

  • Specificity & Efficiency

    Minimized unnecessary reactions such as mis-priming and primer dimer with excellent specificity and amplification efficiency than with other products when compared with same PCR cycles by the use of Hotstart Top DNA polymerase

  • User-friendly

    PCR condition optimization not required with precise specificity of the product only requiring addition of template DNA and primers for anybody to simultaneously amplify the target DNA

  • Stability

    Enhanced stability compared to solution-type products allowing delivery at room temperature by addition of stabilizing agents

  • Reproducibility

    Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables through the mass production under ISO 9001 quality system

Application

• STR analysis

• Molecular diagnostic analysis

• Qualitative, semi-qualitative gene expression assay

• Mutant screening

• Transgenic organism analysis

• Genotyping assay

Experimental data

figure3

Figure. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.
10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).

Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl2).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 65°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer)

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