AccuPower® RT PreMix contains premixed dried components essential for cDNA synthesis such as reverse transcriptase and RNase inhibitor, allowing RT reaction to simply start by just adding the RNA, primers, and D.W. Furthermore, by using the RNase H+ of M-MLV Reverse Transcriptase, template RNA is removed after the cDNA synthesis to minimize the effect of residual template RNA in PCR reaction.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※MasterMix product is shipped in dry ice.
※ A separate purchase of RT Primer is needed, since this product does not contain RT primer (Oligo dT, Random primer).
Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests easily simply by adding template DNA and primers
Addition of stabilizer and RNase inhibitor for high resistance to degradation
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables
• First-strand synthesis of cDNA from RNA molecules (RT)
• Random priming reaction
• cDNA library construction
• Probe labeling
• mRNA 5’-end mapping by primer extension analysis
• PCR
• Real-time qPCR
Figure. Amplification of an GAPDH target gene was detected using human total RNA (from 10 ng to 10 pg ) with AccuPower® RT PreMix.
Lanes 1-4; 10 ng, 1 ng ,and 100 pg, 10 pg of total RNA from HeLa cells, respectively.
