AccuPower® Pfu PCR PreMix & Master Mix
AccuPower® Pfu PCR PreMix uses Pfu DNA polymerase to attain highly accurate PCR products through its proof-reading function. As all components necessary for PCR such as Pfu DNA polymerases, dNTPs, reaction buffers, etc., are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run, by simply adding the template DNA and primers, a high-performance PCR product can be obtained.
※Master Mix product is shipped in dry ice.
Features and Benefits
- High fidelity
Low mutation rate during DNA amplification through its a high fidelity (error rate= 1.9X10-6)
- Sensitivity
Excellent sensitivity and amplification efficiency by detecting even a trace amount of samples
- Long range PCR
Effective amplification of large-size targets allowing various applications such as promoter assay and cloning experiments
- Stability
Enhanced stability allowing long-term storage with a stabilizer included and dried in the PCR reaction mixture
- User-friendly
Convienance with easy start just by adding template DNA and primers
- Reproducibility
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variable by mass production under ISO 9001 quality system
Application
• Gene synthesis
• Gene cloning
• Conventional PCR
• Primer extension
• Site-directed mutagenesis
• High fidelity PCR
Experimental data
Figure. Template Range & Sensitivity test
Test of working range & sensitivity of AccuPower® Pfu PCR PreMix for Lambda DNA template.
M; 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Line 1; 10ng
Line 2; 1ng
Line 3; 100pg
Line 4; 10pg
Line 5; 1pg
Line 6; 100 fg
Line 7; 10 fg
Line 8; template negative
