Rocketscript™ Reverse Transcriptase͵ RNase H Minus
This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.
※This product is shipped in dry ice.
Features and Benefits
- Long cDNA Synthesis
Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H
Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products
Excellent amplification sensitivity even with a trace amount of 1 pg RNA
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
- Gene synthesis
First-strand synthesis of cDNA from RNA molecules (reverse transcription)
Random priming reactions
mRNA 5’-end mapping by primer extension analysis
Figure. Amplification results of RocketScript ™ Reverse Transcriptase, RNase H Minus. (A) Sensitivity (B) Thermostability.
Lane M; 100 bp DNA Ladder(Bioneer, Cat. No. D-1030), Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell, Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell, Lane 5; 10 pg human total RNA from HeLa cell