Rocketscript™ Reverse Transcriptase͵ RNase H Minus

This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.

※This product is shipped in dry ice.

As low as $111.00
Cat. No.

Features and Benefits

  • Long cDNA Synthesis

    Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H

  • Thermostability

    Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products

  • Sensitivity

    Excellent amplification sensitivity even with a trace amount of 1 pg RNA 

  • Reproducibility

    Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system

Application

  • Gene synthesis
  • First-strand synthesis of cDNA from RNA molecules (reverse transcription)

  • Random priming reactions

  • Library construction

  • Probe labeling

  • mRNA 5’-end mapping by primer extension analysis

  • Real-time PCR

  • RT-PCR

Experimental data


Figure. Amplification results of RocketScript ™ Reverse Transcriptase, RNase H Minus. (A) Sensitivity (B) Thermostability.

Lane M; 100 bp DNA Ladder(Bioneer, Cat. No. D-1030), Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell, Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell, Lane 5; 10 pg human total RNA from HeLa cell

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