Rocketscript™ Reverse Transcriptase

RocketScript^TM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA.

※This product is shipped in dry ice.

As low as $110.00

Cat. No.

Product Overview

Features and Benefits

Thermostablity
Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions
User-friendly
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
Reproducibility
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system

Application

Gene synthesis
First-strand synthesis of cDNA from RNA molecules (reverse transcription)
RT-PCR
Random priming reactions
Library construction
Probe labeling
mRNA 5'-end mapping by primer extension analysis
Real time PCR

Experimental data

Figure. Complex RNA amplification.
Complex RNA amplification results of RocketScript™ Reverse Transcriptase compared with that of conventional RTase.
Reverse transcription condition: conventional 42°C/50°C/60°C 1 hr, deactivation 95°C 5 min, This product shows thermal stability.

Lane A; M-MLV Reverse Transcriptase
Lane B; RocketScript™ Reverse Transcriptase
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell

Technical/Specs

▶ Specifications

5' to 3' exonuclease	No
3' to 5' exonuclease	No
3' – A overhang	No
Fragment size	Up to 10 kb

▶ Components

• 5x Reaction Buffer: Tris-HCl, KCl, MgCl₂, Stabilizers (pH8.5)

• 100 mM DTT

• 10 mM dNTPs mix: 2.5 mM of each dNTP

▶ Concentration

10,000 Units (200 U/μl)

▶Storage Conditions

50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers.

▶ Storage Temperature

-20°C

▶ Experimental data

Figure 1. Thermostable Stability Check.
Amplification results of RocketScript™ Reverse Transcriptase using myc compared with supplier's reverse transcription condition: incubation at each temperature 45/50/55/60/65/70 °C for 1 hr, deactivation at 95°C for 5 min

Primer set: human myc 495 bp set

Lane M; 1 kb DNA Ladder(Bioneer, Cat. No. D-1040)
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell

Figure 2. Comparison of amplification quality between RocketScript™ Reverse Transcriptase and competitor Reverse Transcriptase.
Sensitivity test. Target gene expression Level.

Lane M; 1 kb DNA Ladder
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell

Literature/Support

◈ Manual

• Manual_RocketScript ™ Reverse Transcriptase

◈ MSDS

• MSDS_5X Reaction buffer for RocketScript ™ Reverse Transcriptase

• MSDS_10 mM dNTPs

• MSDS_100 mM DTT

• MSDS_RNase Inhibitor

• MSDS_RocketScript ™ Reverse Transcriptase

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option	Price
E-3141	Rocketscript ™ Reverse Transcriptase	10,000 U (50 rxn)	$E-3141
E-3142	Rocketscript ™ Reverse Transcriptase	50,000 U (250 rxn)	$E-3142