Rocketscript™ Reverse Transcriptase

RocketScriptTM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA.

※This product is shipped in dry ice.


As low as $110.00
Cat. No.

Features and Benefits

  • Thermostablity

    Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions

  • User-friendly

    Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.

  • Reproducibility

    Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system

Application

  • Gene synthesis
  • First-strand synthesis of cDNA from RNA molecules (reverse transcription)
  • RT-PCR
  • Random priming reactions
  • Library construction
  • Probe labeling
  • mRNA 5'-end mapping by primer extension analysis
  • Real time PCR

Experimental data

figure1figure2

Figure. Complex RNA amplification.
Complex RNA amplification results of RocketScript™ Reverse Transcriptase compared with that of conventional RTase. 
Reverse transcription condition: conventional 42°C/50°C/60°C 1 hr, deactivation 95°C 5 min, This product shows thermal stability.

Lane A; M-MLV  Reverse Transcriptase
Lane B; RocketScript Reverse Transcriptase
Lane 1; 100 ng human total RNA from HeLa cell   
Lane 2; 10 ng human total RNA from HeLa cell

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