Rocketscript™ Reverse Transcriptase
RocketScriptTM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA.
※This product is shipped in dry ice.
Features and Benefits
Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
- Gene synthesis
- First-strand synthesis of cDNA from RNA molecules (reverse transcription)
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5'-end mapping by primer extension analysis
- Real time PCR
Figure. Complex RNA amplification.
Complex RNA amplification results of RocketScript™ Reverse Transcriptase compared with that of conventional RTase.
Reverse transcription condition: conventional 42°C/50°C/60°C 1 hr, deactivation 95°C 5 min, This product shows thermal stability.
Lane A; M-MLV Reverse Transcriptase
Lane B; RocketScript™ Reverse Transcriptase
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell