Tfi DNA Ligase

Tfi DNA Ligase binds between 5'-phosphate terminal and the 3'-hydroxyl terminal of to the truncated double-stranded DNA molecules with a phosphodiester bond. As the reaction temperature between 45 ° C and 65 ° C,  its activity can be kept especially stable even at higher temperature than other T4 DNA ligases and E. coli DNA ligases.

※This product is shipped in dry ice. 

As low as $75.00
Cat. No.

Features and Benefits

  • Thermostability

    High resistance to heat allow stable reactions than T4 DNA Ligase, E. coli DNA ligase

  • Reproducibility

    Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system

Experimental data

Figure 1. Ligation test at various temperatures (45℃ ~ 65℃)

Incubate the reaction containing ligase 1 unit and 1 ug DNA [lambda/PspE I] at each temperature for 10 min.

- Lane 1: λ DNA/PspE I (control)             - Lane 2: Incubate at 45℃, 10 min
- Lane 3: Incubate at 50℃, 10 min         - Lane 4: Incubate at 55℃, 10 min
- Lane 5: Incubate at 60℃, 10 min         - Lane 6: Incubate at 65℃, 10 min

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