Homology-directed repair (HDR) is a major DSB repair system, capable of performing gene knock-in/out, replacement, and point mutations.
The Knock-in, which inserts a target gene into a specific part of the genome, restores DSB by inserting a donor template containing a homologous sequence, so precise genome editing is possible through the HDR system.
The donor template used here contains homology arms on either side of the insertion (or desired to be modified) sequence.
AccuTool™ Donor synthesis service is available in either double-stranded form (dsDNA Donor) or single-stranded form (ssDNA Donor).
The efficiency of the HDR system may vary by target DNA region, donor template, etc. (Figure 1).
| ssDNA Donor | dsDNA Donor |
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Figure 1. HDR pathway uses homologous donor template (dsDNA Donor or ssDNA Donor) to be inserted accurately.
• Donor Synthesis Service
ssDNA or dsDNA can be synthesized according to the customer’s request. Donor templates can be customized and ordered from BIONEER's Donor Design Service.
For researchers having trouble, BIONEER designs the donor templates with high knock-in efficiency.
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