BIONEER provides In/del analysis service and Mutation Detection Kit based on the next generation sequencing (NGS) data to validate its genome editing efficiency.
AccuCRISPR™ In/del analysis service analyzes a specific genome site using targeted resequencing technique based on NGS, which can quickly get results at a reasonable price. Therefore, it is suitable for testing the genome editing efficiency of CRISPR-Cas9 (Figure 1).
The NGS results will be provided in the form of data reports, including the raw data. Those will be sent to the e-mail address written in the order form.
Only Mi-seq running will be done for the validation test. At least 1 plate (96 samples) must be requested for this service. Send us the prepared samples that are ready to be run, along with their accurate index and adaptor information. The results will be provided in the FASTQ file format.
Figure 1. Workflow for In/del analysis service & Only Mi-seq running
This kit includes a T7E1 enzyme and positive controls that are mostly used in genotyping.
We provide 80 or more of Q30 (%) score.
We provide total reads of at least 10,000.
Our data reports are highly organized, making them easier to read.
This kit contains all the products necessary for the mutation detection.
Genome editing results can be easily confirmed through in vitro methods.