AccuTool™ Custom Design service

Please sign in before filling out the form. Otherwise, the data will not be saved.

• gRNA design service(NHEJ, KO) ATC-0001

Target organism Some target organisms may not be serviceable*
Target Gene Gene name / Gene ID / NCBI or Ensembl Accession#*
Comments

• Donor design service(HDR, KI, PM) ATC-0002

Target organism Some target organisms may not be serviceable*
Target Gene Gene name / Gene ID / NCBI or Ensembl Accession#*
Guide RNA sequence Leave it blank for gRNA design service
Insertion sequence*
Comments

AccuTool™ gRNA & Donor Design Service provides designs of gRNA and Donor template optimized for an efficient CRISPR-Cas9 genome editing.

gRNA Design: The most important part of CRISPR-Cas9 Technology

Designing accurate gRNA is the key to successful genome editing using the CRISPR-Cas9 system.
gRNA is a single-stranded RNA composed of tracrRNA, which forms a complex with Cas9 nuclease, and a 20-nt crRNA that binds to the target DNA region with complementary base pairing (Figure 1).
Simply altering the target sequence of gRNA can change the active region of Cas9 nuclease.
Therefore, designing and synthesizing gRNA is the most fundamental step in CRISPR-Cas9 experiments.

Figure 1. gRNA and Cas9 nuclease
A stem-loop structure of tracrRNA and the target-specific crRNA form a complex with Cas9 nuclease to perform a genome editing.

  • gRNA Design Service
    For researchers having difficulties with designing the gRNA, our gRNA design service can provide 3 to 4 target sequences having the best genome editing efficiency.
    To maximize the accuracy and fidelity of CRISPR, we check up to 3 bp mismatches.

Features and Benefits

  • Optimized gRNA design
    BIONEER checks up to 3 bp mismatches and provides the most effective target sequences.
  • Quick turnaround time
    BIONEER starts the design service as soon as the order is confirmed, and quickly sends the desired results.

Donor designs are the key for a successful knock-In experiment

Homology-directed repair (HDR) is a major DSBs repair system, capable of performing gene knock-in/out, replacement, and point mutations.
The Knock-in, which inserts a target gene into a specific part of the genome, restores DSBs by inserting a donor template containing a homologous sequence, so precise genome editing is possible through the HDR system.

  • Donor Design Service
    For researchers having difficulties with designing the donor, our Donor design service can provide the donor template sequences having the best knock-in efficiency.
    You may order this donor template sequence in the form of ssDNA or dsDNA.

Features and Benefits

  • Optimized donor design
    Our experts provide the best donor template design for your researches.
    • Quick turnaround time
      BIONEER starts the design service as soon as the order is confirmed, and quickly sends the desired results.

    Copyright© 2022 BIONEER CORPORATION. All rights reserved.