AccuTool™ gRNA & Cas9 (Plasmid)

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• dRGEN Synthesis (sgRNA plasmid)

Custom dRGEN synthesis         sgRNA plasmid (1~2 μg) ATC-0050         sgRNA plasmid (50 μg) ATC-0051
sgRNA-GFP plasmid (1~2 μg) ATC-0052 sgRNA-GFP plasmid (50 μg) ATC-0053
Positive control dRGEN synthesis EGFP - sgRNA Plasmid (2 μg) ATS-0054 CCR5 - sgRNA Plasmid (2 μg) ATS-0055 HPRT1 - sgRNA Plasmid (2 μg) ATS-0056

• pRGEN Cas9-Customized vector

CMV/T7 CMV/T7-Puro-RFP CMV/T7-Hygro-EGFP Ef1α Ef1α-Puro-RFP Ef1α-Hygro-EGFP
Cas9 (Wild Type)   5 μg ATS-0060   5 μg ATS-0062   5 μg ATS-0064   5 μg ATS-0066   5 μg ATS-0068   5 μg ATS-0070
50 μg ATS-0061 50 μg ATS-0063 50 μg ATS-0065 50 μg ATS-0067 50 μg ATS-0069 50 μg ATS-0071
Nickase(D10A) Cas9   5 μg ATS-0072   5 μg ATS-0074   5 μg ATS-0076   5 μg ATS-0078   5 μg ATS-0080   5 μg ATS-0082
50 μg ATS-0073 50 μg ATS-0075 50 μg ATS-0077 50 μg ATS-0079 50 μg ATS-0081 50 μg ATS-0083
Sniper Cas9   5 μg ATS-0084   5 μg ATS-0086   5 μg ATS-0088   5 μg ATS-0090   5 μg ATS-0092   5 μg ATS-0094
50 μg ATS-0085 50 μg ATS-0087 50 μg ATS-0089 50 μg ATS-0091 50 μg ATS-0093 50 μg ATS-0095

• Smallest size Cas9 from campylobacter jejuni

dRGEN_CjCas9 (sgRNA plasmid) 1~2 μg ATC-0100 50 μg ATC-0101
pRGEN_CjCas9_CMV      5 μg ATS-0102 50 μg ATS-0103

Plasmid DNA-based RNA-Guided Endonuclease (dRGEN) is a new generation of engineered nuclease based on the CRISPR-Cas9 system. It is an innovative tool for genome editing composed of Cas9 nuclease and gRNA. By recognizing the target sequence (19-20 bp) and PAM sequence (5'-NGG-3'), dRGEN can easily edit any genomic regions.
The target DNA region can be effectively cleaved during either in vivo or in vitro genome editing applications (knock-in/out, and locus deletion).

Plasmid DNA-directed Cas9 protein & sgRNA

AccuTool™ gRNA & Cas9 (Plasmid) service is a plasmid-based genome editing system that allows researchers to edit the genomes in an efficient, effortless way.
It provides both custom sgRNA expression plasmid, with GFP plasmid as an optional choice, and human codon optimized Cas9 expression plasmid.
sgRNA-GFP expression plasmid allows you to confirm the activity level in cells by fluorescent microscope. Three Cas9 expression plasmids are available to be chosen: WT/Nickase/Sniper types.
They can be delivered to your cell of interest by any standard methods like lipofection, nanoparticle, or electroporation to achieve highly efficient delivery.

  • sgRNA plasmid (dRGEN) 

    AccuTool™ dRGEN service synthesizes gRNA according to the customers' requests. They can choose to insert it into either sgRNA plasmid or sgRNA-GFP plasmid. Furthermore, dRGEN positive controls targeting EGFP, CCR5, and HPRT1 genes are provided.
    They can be transfected directly into your cell of interest without any pre-treatment steps.

    dRGEN synthesis sgRNA plasmid
    sgRNA-GFP plasmid
  • pRGEN Cas9-Customized vector
    Cas9 (Nickase)/
    Sniper Cas9
    CMV/T7 Basic
    Puro-RFP
    Hygro-EGFP
    Ef1α Basic
    Puro-RFP
    Hygro-EGFP
  • Smallest size Cas9 from Campylobacter jejuni

    CjCas9 is derived from Campylobacter jejuni (C. jejuni) and is the smallest Cas9 orthologue (2.9 kb), which improves upon the disadvantages of large-sized SpCas9 (4 kb), derived from Streptococcus pyogenes (S. pyogenes).
    The small size of CjCas9 allows usage in clinical studies.

Features and Benefits

  • Wide usage

    BIONEER provides the gRNA of your desired sequence and the dRGEN positive controls (EGFP, CCR5, and HPRT1 gene).
    The plasmid type allows the products to be applied in a wide variety of researches.

  • Easy and simple to use

    Easily create a cell-line with your desired selection marker and deliver the products into your cell of interest with chemical transfection or electroporation.

  • Economical & Fast Delivery

    Get your products quickly at a reasonable price.

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