AccuPower® Theileria PCR Kit

AccuPower® Theileria PCR Kit is designed to detect 16 species of Theileria genus from blood of Theileria-infected livestock.

※ This product is shipped in dry ice.

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Cat. No.

THE-1115P

Product Overview

Theileria, which is parasitic on red blood cells in human or animals, has been mainly distributed in trophical and subtrophical region so far, but shows also increasing global distribution by the effect of global warming. Theileria-related disease is distributed globally in livestocks. It is mediated by 3 kinds of ticks in their hosts, which are found in Korea also. It shows 70-100% of significant ratio of infection in Korea, and 30-50% of that of proceeding into disease. Once-infected animal may mediate infection of those microbials to other animals, and would give huge damage to livestock farmers. Theileria-infected human or animal shows symptons including anemia by hemolysis, fatigue, loss of appetite, headache, high fever and chill at 1-4 weeks after infection. It may result in fatality if an immune suppressed animal is infected.

	AccuPower^® Theileria PCR Kit	Animal Disease Standard Diagnostic Method (QIA*)	G company (Germany)	B company (Chile)
Theileria	Detection of 16 species of Theileria with ~ 10 copies of sensitivity	Detection of 1 species (T. Sergenti only)	Single detected (T.annulata) Theileriagenus kit	Single detected (T.annulata, T.ovis, T.lestoquardi,T.parva) Theileriagenus kit

* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea

Features

Developed cooperatively with QIA(Quarantine and Inspection Agency), Application of patent completed (Patented).

> High performance
Detection of 16 kinds of Theileria

Theileria Detected

Theileria annulata, Theileria buffeli, Theileria cervi, Theileria equi, Theileria lestoquardi, Theileria mutans, Theileria orientalis, Theileria ovis, Theileria parva, Theileria separata, Theileria sergenti, Theileria sinensis, Theileria taurotragi, Theileria uilenbergi, Theileria velifera, and Theileria youngi

> World-class sensitivity and specificity
Provides high sensitivity by Chemical-Mediated HotStart method to detection limit of 10 copies of Theileria sergenti.

> Great reproducibility
Dried-type HotStart PCR PreMix type – Addition of template DNA and DW only required & highly reproducible result provided.

> Prevention of amplicon-induced cross-contamination by 8-MOP
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.

> High Stability
1-year warranty under -20 °C.

> Quality Control

All AccuPower® products are manufactured under ISO 9001 Quality Control System.

Technical/Specs

Specifications

Instrument	AllInOneCycler™ Thermal Block or other Thermal Block
Specimen	Whole blood
Time to result	1h 25min (Post-extraction)
Tests per Kit	96

Performances

Figure 1. Sensitivity and Specificity of AccuPower® Theileria PCR Kit.
(A) Target gene of Theileria sergenti were used for the sensitivity test (lane 1 - 5 : 1.0 x 10⁵ - 1.0 x 10¹ copies, serial 10-fold dilution). PCR condition was as follows: 95°C for 5 min, 95°C for 20 sec, 65°C for 35 sec (40 cycles), then 72°C for 5 min. 5 ul of PCR product were loaded on 2% TBE Agarose gel, containing Ethidium bromide (EtBr), followed by electrophoresis (30-40 min).
(B) Target gene of 2 Babesia species (Babesia gibsoni & Babesia ovata, lane B1 & B2) and Bovine or Dog normal blood were used for specificity test. Sample 6, 7, B1 and B2 were prepared and used as 1.0 x 10⁵ copies/reaction. PCR condition was as follows: 95°C for 5 min, 95°C for 20 sec, 65°C for 35 sec (40 cycles), and 72°C for 5 min. 5 ul of PCR product were loaded on 2% TBE Agarose gel, containing EtBr, followed by electrophoresis (30-40 min).

Figure 2. Sensitivity test.

Comparison of sensitivity between AccuPower® Theileria PCR Kit (A) and Standard methods for Theileria sergentiprimer set (B, Animal, Plant and Fisheries Quarantine and Inspection Agency). PCR condition was as follows; A: 95°C 5 min, 95°C 20 sec, 65°C 35 sec (35 cycles), and 72°C 5 min. / B : 95°C 15 min, 96°C 30 sec, 60°C 1 min, 72°C 1 min (35 cycless), and 72°C 10 min]. 5 ul of PCR product were loaded on 2% TBE Agarose gel, containing EtBr, followed by electrophoresis (30-40 min).

Ordering Info

Ordering information

Cat. No.	Product Description	Price
THE-1115P	AccuPower^® Theileria PCR Kit	Inquire

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Quality assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate