AccuPower® Babesia PCR Kit

AccuPower® Babesia PCR Kit is designed to detect 17 species of Babesia genus from blood of Babesia-infected livestock.

※ This product is shipped in dry ice.

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Cat. No.

BAB-1115P

Babesia, which is parasitic on red blood cells in human or animals, has been mainly distributed in trophical and subtrophical region so far, but shows also increasing global distribution by the effect of global warming.Babesia-related disease is distributed globally in livestocks. It is mediated by 3 kinds of ticks in their hosts, which are found in Korea also. Once-infected animal may mediate infection of those microbials to other animals, and would give huge damage to livestock farmers. Especially patients who were infected by B. bovine, B. divergens or B. microti were reported in Korea on 1990. Babesia-infected human or animal shows symptons including anemia by hemolysis, fatigue, loss of appetite, headache, high fever and chill at 1-4 weeks after infection. It may result in fatality if an immune suppressed animal is infected.

	AccuPower^® Babesia PCR Kit	Animal Disease Standard Diagnostic Method (QIA*)	G company (Germany)	I company (Korea)	B company (chile)
Babesia	Detection of 17 kinds of Babesia, ~10 copies	Detection of 2 types (B. bovine, B. bigemina)	Single detected (B.bigemina, B.bovis, B.caballi, B.euai, B.gibsoni)	Detection of 2 types (B. canis, B. gibsoni)	Single detected (B.canis, B.gibsoni, B.ovis, B.caballi, B.bigemina, B.bovis, B.pullorum)

* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea

Features

Developed cooperatively with QIA (Quarantine and Inspection Agency), Application of patent completed (Patented).

>High performance
Detection of 17 kinds of Babesia

Babesia Detected

Babesia bennetti, Babesia bigemina, Babesia bovis, Babesia caballi, Babesia canis, Babesia crassa, Babesia conradae, Babesia divergens, Babesia gibsoni, Babesia lengau, Babesia major, Babesia motasi, Babesia occultans, Babesia odocoilei, Babesia ovata, Babesia ovis, Babesia rodhaini

> World-class sensitivity and specificity
Provides high sensitivity by Chemical-Mediated HotStart method. High sensitivity to detection limit of 10 copies of Babesia ovate.

> Great reproducibility
Dried-type HotStart PCR PreMix type – Addition of template DNA and DW only required & highly reproducible result provided.

> Prevention of amplicon-induced cross-contamination by 8-MOP
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.

> High Stability
1-year warranty under -20 °C.

> Quality Control

All AccuPower® products are manufactured under ISO 9001 Quality Control System.

Technical/Specs

Specifications

Instrument	AllInOneCycler™ Thermal Block or other Thermal Block
Specimen	Whole blood
Time to result	1h 45min (Post-extraction)
Tests per Kit	96

Performances

Figure 1. Sensitivity and Specificity of AccuPower® Babesia PCR Kit.
(A) Target gene of Babesia ovata were used for the sensitivity test (lane 1-5 : 1.0 x 10⁵ - 1.0 x 10¹ copies, serial 10-fold dilution). PCR condition was as follows: 95°C for 5 min, 95°C for 20 sec, 65°C for 1 min (40 cycles), then 72°C for 5 min.
5 ul of PCR product were loaded on 2% TBE Agarose gel, containing Ethidium bromide (EtBr), followed by electrophoresis (30-40 min).
(B) Target gene of 2 Theileria species (Theileria sergenti & Theileria equi, lane T1 & T2) and Bovine or Dog normal blood were used for specificity test. Sample 6, 7, T1 and T2 were prepared and used as 1.0 x 10⁵ copies/reaction. PCR condition was as follows: 95°C for 5 min, 95°C for 20 sec, 65°C for 1 min (40 cycles), and 72°C for 5 min. 5 ul of PCR product were loaded on 2% TBE Agarose gel, containing EtBr, followed by electrophoresis (30-40 min).

Figure 2. Comparison between AccuPower® Babesia PCR Kit (A) and Babesia canis & B. gibsoni Detection kit (B, I company).
PCR condition was as follows; A: 95°C for 5 min., 95°C for 20 sec, 65°C for 1 min (40 cycles), and 72°C for 5 min. / B : 95°C for 5 min., 95°C for 30 sec, 65°C for 30 sec, 72°C for 40 sec (40 cycles), and 72°C for 5 min. 5 ul of PCR product were loaded on 2% TBE Agarose gel, containing EtBr, followed by electrophoresis (30-40 min). Positive control (P) is Babesiosis (Babesia species), 1.0 x 10⁵ copies. In order to check specificity of these two kits, Theileria species (Theileria sergenti and Theileria equi) and Bovine or Dog normal blood were used (1.0 x 10⁵ copies of genes).

Figure 3. Sensitivity test.
Comparison of sensitivity between AccuPower® Babesia PCR Kit (A) and Babesia canis & B. gibsoni Detection kit (B, I company) PCR condition was as follows; A: 95°C 5 min, 95°C 30 sec, 65°C 1 min (40 cycles), and 72°C 5 min. / B : 95°C 5 min, 95°C 30 sec, 65°C 30 sec, 72°C 40 sec (40 cycles), and 72°C 5 min. 5 ul of PCR product were loaded on 2% TBE Agarose gel, containing EtBr, followed by electrophoresis (30-40 min).

Ordering Info

Ordering information

Cat. No.	Product Description	Price
BAB-1115P	AccuPower^® Babesia PCR Kit	Inquire

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Quality assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate