AccuPower® qPCR Array System: Reference qPCR primer Set
AccuPower® qPCR Array System: Reference qPCR primer set consists of 16 housekeeping genes including representative cell function regulatory gene which is devided 8sets of A and 8sets of B. And by ensuring uniform expression of genes under various experimental conditions, relative quantitative analysis can be performed easily.
Reference gene (internal control) is crucial for cell survival known to be constantly expressed in all tissues. However, its expression level may vary depending on the experimental conditions, cell type, etc.
Figure 1. ACTB expression levels in the various tissues. (biogps.org)
Features and Benefits
16 kinds of commonly used reference genes (A set: 8 genes / B set: 8 genes) possible to be screened
qPCR primer with qPCR efficiency of 90 - 110% ensuring detection limit of 100 copies.
Reduced time and cost for primer design, synthesis and efficiency assays
- Quantitative Real-time PCR