AccuPower® Ligation PreMix (96 T, 20 μl)
AccuPower® Ligation PreMix is freeze-dried in a 0.2 ml tube by mixing components necessary for ligation reactions such as T4 DNA Ligase, ATP and reaction buffer. DNA can be ligated to the plasmid vector with high efficiency within 5 minutes at the room temperature (10 minutes for Blunt-end DNA). Moreover, stabilizers added to keep the activity at room temperature for 4 months. If kept frozen (-20°C), the activity will remain constant for more than 3 years.
※This product is shipped in dry ice.
Features and Benefits
- Fast reaction rate
Ligation only 5 minutes for cohesive ends DNA and 10 minutes for blunt ends DNA at room temperature
Enhanced stability with enzyme activity for up to four months at room temperature and for three years in the -20℃ by the additon of a stabilizer
Simplified procedures with all componetns necessary for T4 DNA Ligase, ATP, and optimized reaction buffer already mixed in 0.2 ml tubes to initate the reactions simply by adding vector and insert DNA
Various applications including cohesive and blunt end DNA ligation reactions
Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product
• Cloning into vectors
• Library construction
• TA cloning
• Linker ligation
• Recirclization of linear DNA