AccuPower®GreenStar™ RT-qPCR PreMix & Master Mix can accurately amplify RNA in various samples by applying Thermostable RTase and HotStart PCR technology. This product has high selectivity capable of undergoing cDNA synthesis even when the target RNA is present in a small amount, making it suitable for various RNA virus tests and gene expression quantitative analysis. Moreover, master mix products are compatible with wide range of real-time PCR instruments.
※This product is shipped in dry ice.
Amplification of target genes present in a miniscule amount of 1 pg template RNA
Minimized experimental errors by non-specific amplification and effective amplification of template RNA existing in a small amount through the use Hotstart Taq DNA polymerase, Thermostable RTase, and Hotstart PCR
Comprehensive choice of template RNA for RT-qPCR reactions, even in a form of strong secondary structure, through the use of Thermostable RocketScript ™ RTase, capable of performing RT reaction at high temperature
Simplified procedure with all components necessary for one-step RT-qPCR such as DNA polymerase, thermostable RTase, reaction buffer, dNTPs, etc. RT-qPCR already mixed in tubes to easily start just by adding the primer, template RNA of the gene to be amplified and D.W.
Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide
• Low copy viral/bacterial pathogen load determination in an earlier stage
• Low copy mRNA amplification
• Low copy target RNA quantification
• RNA amplification for microarray and/or for NGS
Figure. High sensitivity of AccuPower® GreenStar™ RT-qPCR PreMix
One-step RT-qPCR reactions were performed with HeLa cell total RNA (from 1 pg to 100 ng) using AccuPower® GreenStar™ RT-qPCR PreMix on Exicycler™ 96 Real-time Quantitative Thermal Block. Target genes were MYC (a), TMS4 (b) and CTN1 (c).