MicroBiome Assay Taq DNA Polymerase

While most of the Taq DNA polymerase products have high chance of being contaminated by host cell E. coli genomic DNA, our MBA (MicroBiome Assay) Taq DNA has been purified with our own technology, which can minimize the host cell gDNA contamination and be effectively used for detection of microorganisms with 16s rRNA specific primers.

※ This product is shipped in dry ice.

$198.00

Cat. No.

E-3504

Product Overview

Features and Benefits

Minimized DNA contamination
- Reduced false positives caused by host cell DNA contamination and other errors from unwanted PCR products during microbial PCR assay
High sensitivity and amplification efficiency
- Optimized for microbial PCR assay and 16s rRNA researches by using the innovative purification technology developed by BIONEER to minimize host cell DNA contamination.

Application

Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
Gene Expression profiling
Microbial & Viral pathogen detection

Technical/Specs

▶ Product specification

5' to 3' exonuclease activity	Yes
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment size	Up to 8 kb

▶ Experimental Data

Figure 1. Limit of Detection of MicroBiome Assay
Data comparing the extent of E.coli gDNA contamination and the limit of detection between the conventional commercial Taq DNA Polymerase and MicroBiome Taq DNA Polymerase using the real-time PCR (45 cycles); 10-fold serial dilution (107~101 fg) is done to E. coli gDNA (template and non-template DNAs).

Figure 2. Gel photos of MicroBiome Assay Taq DNA polymerase showing high sensitivity and low contamination of E.coli gDNA.
Experiments done to check the sensitivity and the strain DNA (E. coli gDNA) contamination of MicroBiome Assay Taq DNA polymerase (35 cycles)

Lane 1; E.coli gDNA 100 pg
Lane 2; E.coli gDNA 10 pg
Lane 3; E.coli gDNA 1 pg
Lane 4; E.coli gDNA 100 fg

Lane N; Non-Template

Figure 3. Gel photos of MicroBiome Assay Taq DNA polymerase showing high sensitivity and low contamination of E.coli gDNA.
Experiments done to check the sensitivity and the host cell DNA (plasmid) contamination of MicroBiome Assay Taq DNA polymerase (35 cycles)

Lane 1; Plasmid DNA 10 pg
Lane 2; Plasmid DNA 1 pg
Lane 3; Plasmid DNA 100 fg
Lane 4; Plasmid DNA 10 fg

Lane N; Non-Template

Literature/Support