MagListo™ 5M Forensic Sample DNA Extraction Kit (100 reactions)

MagListo™ Forensic Sample DNA Extraction Kit allows rapid extraction of total DNA using Magnetic Nanobead and MagListo™ Magnetic Separation Rack from various forensic samples with ease. The isolated DNA can be used for a wide range of experiments such as PCR, quantitative real time PCR, SNP genotyping and STR analysis.

Magnetic bead type

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Cat. No.

K-3615

Product Overview

Features and Benefits

Quick & Comprehensive purification
Utilization of Magnetic Nanobead for total DNA extraction from a broad range of forensic samples (whole blood, saliva, urine, fingerprints, hair, nails, bones, etc.)
Convenience
No need of additional expensive instruments other than the kit and MagListo™ Magnetic Separation Rack

Application

• PCR

• Real-time PCR

• SNP genotyping

• STR analysis

Technical/Specs

▶ Specifications

Feature	Specification
Sample type	Various kind of forensic sample
Technology	Magnetic nano bead
Hands-on time	< 10 min
Expected purity	A_260/280 > 1.8

* Differences in the quantified value may occur depending on the types of samples.

▶ Components

Components	K-3615
TL Buffer (Lysis)	35 ml x 1 ea
GB Buffer (Binding)	30 ml x 1 ea
WM1 Buffer (1st Washing)	40 ml x 1 ea
W2 Buffer (2nd Washing)	80 ml x 1 ea
WE Buffer (3rd Washing)	80 ml x 1 ea
EA Buffer (Elution)	15 ml x 1 ea
Poly (A), lyophilized	1 mg x 1 ea
Proteinase K, lyophilized	25 mg x 2 ea
Magnetic Nano Bead - DNA	1.8 ml x 6 ea
User's Guide	1 ea

▶ Experimental data

(A) Urine

Product	Sample volume
Product	10 ml	5 ml	1 ml	NTC
MagListo™	32.79	33.53	35.24	product
MagListo™	32.33	33.08	35.21
Q	32.48	33.36	38.93
Q	32.76	33.08	40.16

(B) Saliva & Blood

Saliva	Kit		Blood	Kit
	MagListo™	Q		MagListo™
1 µl	33.98	33.40	1 µl	31.42
	33.66	33.51		31.32
10 µl	30.55	30.93	10 µl	28.32
	30.44	31.28		28.14
100 µl	26.91	30.93	100 µl	28.32
	27.66	27.64		25.51
NTC	U.D.
	U.D.

(C) Saliva (10 ul) & Blood (10 ul) stains from clothes (7 mm diameter)

Sample	Kit
	MagListo™	Q
Saliva	30.30	32.05
	30.60	32.14
Blood	29.15	29.69
	29.14	29.89
NTC	U.D.
	U.D.

(D) Saliva (10 ul) and fingerprint on paper (7 mm diameter)

Sample	Kit
	MagListo™	Q
Saliva	28.07	28.1
	28.43	28.59
Fingerprint	36.33	36.45
	36.29	36.45
NTC	U.D.
	U.D.

(E) Saliva on cotton swab and chewing gum

Kit	Ct
	Swab	Gum
MagListo™	26.41	32.10
	28.29	32.25
Q	26.87	31.21
	26.45	31.10
NTC	U.D.
	U.D.

(F) Hair 1 strand (25 cm)

Sample	Kit
	MagListo™	Q
w/ root	29.99	30.7
	30.04	30.4
w/o root	33.39	34.66
	33.08	34.22
NTC	U.D.
	U.D.

(G) Nail clipping (15 mg)

Kit	Ct
MagListo™	28.00
MagListo™	27.98
Q	30.04
Q	29.46
NTC	U.D.
NTC	U.D.

Figure 1. Comparison of real-time PCR data of DNA extracted using MagListo™ and those using done using a competitor’s product (single-column type).

Figure 2. Comparison of total DNA from skeletal muscle tissues purified with MagListo™ and that done with a competitor’s kit (single column type).

M; 1 kb Ladder
1 mg
MagListo™; yield: 1.35 ug purity(A_260/280): 1.95
Competitor; yield: 0.57 ug purity(A_260/280): 1.86
5 mg
MagListo™; yield: 5.22 ug purity(A_260/280): 1.90
Competitor; yield: 2.81 ug purity(A_260/280): 1.88

Figure 3. Comparison of DNA extraction time from forensic samples purified with MagListo™ and that done with competitor’s kit (single column type).

N: Number of Samples

Literature/Support