AccuPrep® Viral RNA Extraction Kit (100 reactions)

AccuPrep^® Viral RNA Extraction Kit allows rapid extraction of viral RNA taken from various cell-free samples such as serum, plasma, CSF and urine with ease. Furthermore, poly(A) powder is included in the product for improved efficiency. This product can seperate comprehensive types of viral RNA from HIV, HAV, HCV, enterovirus, etc. The isolated RNA can be used for a wide range of experiments such as RT-PCR and Quantitative Real time RT-PCR, etc.

Spin column type

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Cat. No.

K-3033G

Product Overview

Features and Benefits

Comprehensive sample type
Highly-pure viral RNA extraction from various clinical samples such as serum, blood samples, CSF (Cerebrospinal Fluid), urine, swab, etc.
Poly(A) powder
Maximized RNA degradation protection and binding efficiency
High binding capacity
Utilization of Silica Magnetic Particles developed and produced by Bioneer's proprietary technology

Application

• cDNA synthesis

• RT-PCR

• Quantative real-time RT-PCR

• poly A+ RNA selection

• Northern blot analysis

• Pathogen detection

Technical/Specs

▶ Specification

sample type	Starting amount	Expected yield
Serum	200 µl	>90% recovery
Plasma
CSF
Cell free body fluid
Swab	1 ea

▶Components

Components	K-3033G
VB Buffer (Virus Binding)	50 ml X 1 ea
VW1 Buffer (1^st Washing)	40 ml X 1 ea
RWB2 Buffer (2^nd Washing)	21 ml X 1 ea
ER Buffer (Elution)	20 ml X 1 ea
BSTB Solution	36 ml X 1 ea
Poly (A), lyophilized	2 mg X 1 ea
Proteinase K, lyophilized	25 mg X 1 ea
AccuPrep^® Binding Column-I	100 ea
Collection Tube (for filtration)	100 ea
1.5 ml Tube (for elution)	100 ea
User's Guide	1 ea

▶ Procedure

▶ Experimental Data

accupower

Kit	Bioneer	Competitor I
Ct	37.07	36.63
	36.03	37.17
	35.57	36.29
	35.58	37.13
	36.27	36.37
	35.03	36.14
	35.75	37.00
	36.21	37.18
Average	35.94	36.74
CV	1.69	1.17

accupower

Kit	Bioneer	Competitor I
Ct	38.75	39.69
	39.12	40.32
	37.78	39.84
	38.03	39.68
	38.34	39.30
	37.87	39.33
	37.67	41.26
	39.30	41.15
	38.07	U.D.
	38.75	39.12
	38.07	39.32
	38.41	39.36
Average	38.35	39.85
CV	1.38	1.87

Figure 1. Comparison of real-time RT-PCR data of hepatitis C virus (HCV) RNA in human normal serum using AccuPrep^® Viral RNA Extraction Kit (Bioneer, manual/single column) and a competitor's kit (Competitor I, manual/single column). AccuPower^® HCV Quantitative RT-PCR Kit (HCV-1111) and 1/10 of eluate as a template was used for detection.

Literature/Support