The primers are known to have a great impact in the results of experiments that involve real-time PCR. Thus, they must be designed under meticulous conditions. Our primers and probe designs are done using our state-of-the-art experience and referring to the latest gene information listed in NCBI database RefSeq (Reference Sequence) to amplify high target specificity and reduce the chance of dimerization.
AccuTarget ™ qPCR Screening kit has 84 primer sets in each well of the 96-well plates. 92 genes, including the control, are lyophilized in a single plate. This product can be used easily as the contents are conveniently divided into its standardized size (10X/20X/30X) for it to be used depending on the experimental conditions.
Ex) PCR Plate layout
Design the primers by considering all the necessary conditions for qPCR such as target specificity, Tm value, and the size of PCR products.
Provide validated primer sets by undergoing target-specific amplification test through meticulously designed algorithms and utilizing BLAST to reduce the time and cost caused by wrong designs.
Easy to be used according to the purpose of the experiment by providing standardized size (10X/20X/30X) with conveniently pipetted contents.
Example analysis data for AccuTarget ™ qPCR Screening kit
Ex) Fold change, Scatters plot, Heatmap, etc.