AccuTarget™ qPCR Screening Kit

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The pre-designed primer set allows to quantify mRNA gene expression levels by screening the gene using Real time PCR(Intercalating dye).
The primers were designed using accurate bioinformatics tools to comply with MIQE guidelines and proved to prevent any non-specific amplification in silico conditions.

Cat. No.	Product Description	Option	Price
SH-0000-10	AccuTarget™ Human qPCR Screening Kit	10X	$180.00
SH-0000-20		20X	$250.00
SH-0000-30		30X	$320.00
SM-0000-10	AccuTarget™ Mouse qPCR Screening Kit	10X	$180.00
SM-0000-20		20X	$250.00
SM-0000-30		30X	$320.00

Species

Pathway name & Plate map

Concentration

Add/Remove Rows

Product Overview

Overview

The primers are known to have a great impact in the results of experiments that involve real-time PCR. Thus, they must be designed under meticulous conditions. Our primers and probe designs are done using our state-of-the-art experience and referring to the latest gene information listed in NCBI database RefSeq (Reference Sequence) to amplify high target specificity and reduce the chance of dimerization.

AccuTarget ™ qPCR Screening kit has 84 primer sets in each well of the 96-well plates. 92 genes, including the control, are lyophilized in a single plate. This product can be used easily as the contents are conveniently divided into its standardized size (10X/20X/30X) for it to be used depending on the experimental conditions.

Ex) PCR Plate layout

Features and Benefits

Professional Design
Design the primers by considering all the necessary conditions for qPCR such as target specificity, Tm value, and the size of PCR products.
Accurate, Cost-Saving Service

Provide validated primer sets by undergoing target-specific amplification test through meticulously designed algorithms and utilizing BLAST to reduce the time and cost caused by wrong designs.
Convenient

Easy to be used according to the purpose of the experiment by providing standardized size (10X/20X/30X) with conveniently pipetted contents.

Applications

Gene expression profile
NGS or Microarray validation
Product Quality Check
Cancer Inflammation and cytokine profiling
Stem cells
Signal transduction pathways
Biomarker screening and Validation

Experimental Data

Example analysis data for AccuTarget ™ qPCR Screening kit
Ex) Fold change, Scatters plot, Heatmap, etc.

Technical/Specs

1. Pathway list

A~B	C	D~F
[0001] Adherens Junctions [0002] Adipogenesis [0003] Aging [0004] Allergy and Asthma [0005] Alzheimers Disease [0006] Amino Acid Metabolism I [0007] Amino Acid Metabolism II [0008] AMPK Signaling [0009] Androgen Receptor Signaling Targets [0010] Angiogenesis [0011] Angiogenic Growth Factor [0012] Antibacterial Response [0013] Antifungal Response [0014] Antiviral Response [0015] Apoptosis [0016] Atherosclerosis [0017] Autophagy [0018] Breast Cancer	[0019] cAMP Calcium Signaling PathwayFinder [0020] Cancer Drug Resistance [0021] Cancer Drug Target [0022] Cancer Inflammation and Immunity Crosstalk [0023] Cancer PathwayFinder [0024] Cancer Stem Cells [0025] Cardiotoxicity [0026] Cardiovascular Disease [0027] Cell Cycle [0028] Cell Death PathwayFinder [0029] Cell Junction PathwayFinder [0030] Cell Lineage Identification [0031] Cell Motility [0032] Cell Surface Markers [0033] Cellular Senescence [0034] Cellular Stress Responses [0035] Chemokines and Receptors [0036] Circadian Rhythms [0037] Common Cytokines [0038] Crohns Disease [0039] Cystic Fibrosis [0040] Cytokines and Chemokines [0041] Cytoskeleton Regulators	[0042] Dendritic and Antigen Presenting Cell [0043] Diabetes [0044] DNA Damage Signaling Pathway [0045] DNA Repair [0046] Dopamine and Serotonin Pathway [0047] Drug Metabolism [0048] Drug Metabolism Phase I Enzymes [0049] Drug Metabolism Phase II Enzymes [0050] Drug Transporters [0051] EGF PDGF Signaling Pathway [0052] Embryonic Stem Cells [0053] Endothelial Cell Biology [0054] Epigenetic Chromatin Modification Enzymes [0055] Epigenetic Chromatin Remodeling Factors [0056] Epithelial to Mesenchymal Transition [0057] Estrogen Receptor Signaling [0058] Extracellular Matrix and Adhesion Molecules [0059] Fatty Acid Metabolism [0060] Fatty Liver [0061] Female Infertility [0062] Fibrosis [0063] Focal Adhesions
G~H	I~L	M~N
[0064] GABA and Glutamate [0065] Gap Junctions [0066] Glucocorticoid Signaling [0067] Glucose Metabolism [0068] Glycosylation [0069] GPCR Signaling PathwayFinder [0070] Growth Factors [0071] Heat Shock Proteins and Chaperones [0072] Hedgehog Signaling Pathway [0073] Hematopoiesis [0074] Hepatotoxicity [0075] Hippo Signaling Pathway [0076] HIV Host Response [0077] Homeobox HOX Genes [0078] Huntingtons Disease [0079] Hypertension [0080] Hypoxia Signaling Pathway [0081] Hypoxia Signaling Pathway Plus	[0082] IL6 STAT3 Signaling Pathway [0083] IL6 STAT3 Signaling Pathway Plus [0084] Immunotoxicity [0085] Induced Pluripotent Stem Cells [0086] Inflammasomes [0087] Inflammatory Cytokines and Receptors [0088] Inflammatory Response and Autoimmunity [0089] Innate and Adaptive Immune Responses [0090] Insulin Resistance [0091] Insulin Signaling Pathway [0092] Interferons and Receptors [0093] JAK STAT Signaling Pathway [0094] let-7a Targets [0095] Leukemia [0096] Lipoprotein Signaling and Cholesterol Metabolism [0097] Liver Cancer [0098] Lung Cancer [0099] Lymphoma	[0100] Macular Degeneration [0101] Male Infertility [0102] MAP Kinase Signaling Pathway [0103] Mesenchymal Stem Cells [0104] Mitochondria [0105] Mitochondrial Energy Metabolism [0106] Molecular Toxicology PathwayFinder [0107] mTOR Signaling [0108] Multiple Sclerosis [0109] MYC Targets [0110] Necrosis [0111] Nephrotoxicity [0112] Neurogenesis [0113] Neuronal Ion Channels [0114] Neurotoxicity [0115] Neurotransmitter Receptors [0116] Neurotrophins and Receptors [0117] NFkB Signaling Pathway [0118] NFkB Signaling Pathway Plus [0119] NFkB Signaling Targets [0120] Nitric Oxide Signaling Pathway [0121] Notch Signaling Pathway [0122] Notch Signaling Pathway Plus [0123] Notch Signaling Targets [0124] Nuclear Receptors and Coregulators
O~R	S~T	U~W
[0125] Obesity [0126] Oncogenes and Tumor Suppressor Genes [0127] Osmotic Stress [0128] Osteogenesis [0129] Osteoporosis [0130] Oxidative Stress [0131] Oxidative Stress Plus [0132] p53 Signaling Pathway [0133] Pain Neuropathic and Inflammatory [0134] Parkinsons Disease [0135] Phagocytosis [0136] PI3K AKT Signaling Pathway [0137] Polycomb and Trithorax Complexes [0138] Polycomb and Trithorax Target Genes [0139] Polycystic Kidney Disease [0140] PPAR Targets [0141] Pre Eclampsia [0142] Primary Cilia [0143] Prostate Cancer [0144] Protease Activated Receptor Signaling [0145] Protein Phosphatases [0146] Retinoic Acid Signaling	[0147] Signal Transduction PathwayFinder [0148] Skeletal Muscle Myogenesis and Myopathy [0149] Stem Cell [0150] Stem Cell Signaling [0151] Stem Cell Transcription Factors [0152] Stress and Toxicity PathwayFinder [0153] Synaptic Plasticity [0154] T Cell Anergy and Immune Tolerance [0155] T Helper Cell Differentiation [0156] T Cell and B Cell Activation [0157] Telomeres and Telomerase [0158] Terminal Differentiation Markers [0159] TGFb BMP Signaling Pathway [0160] Human TGFb Signaling Targets [0161] Th1 and Th2 Responses [0162] Th17 Response [0163] Tight Junctions [0164] TNF Signaling Pathway [0165] Toll Like Receptor Signaling Pathway [0166] Transcription Factors [0167] Transplant Rejection [0168] Tumor Metastasis [0169] Type I Interferon Response [0170] Tyrosine Kinases	[0171] Ubiquitination Ubiquitinylation [0172] Unfolded Protein Response [0173] Unfolded Protein Response Plus [0174] VEGF Signaling [0175] WNT Signaling Pathway [0176] WNT Signaling Pathway Plus [0177] WNT Signaling Targets [0178] Wound Healing

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

▶ Order details

Cat. No.	Product Description	Option	Price
SH-0000-10	AccuTarget ™ Human qPCR Screening Kit	10X	$SH-0000-10
SH-0000-20		20X	$SH-0000-20
SH-0000-30		30X	$SH-0000-30
SM-0000-10	AccuTarget ™ Mouse qPCR Screening Kit	10X	$SM-0000-10
SM-0000-20		20X	$SM-0000-20
SM-0000-30		30X	$SM-0000-30

▶Custom order

Cat. No.	Product Description	Option	Price
SPC-0001-10	AccuTarget ™ qPCR Screening Kit-Pathway Custom Kit (Modify the plate design with 1~5 genes)	10X	Inquire
SPC-0001-20		20X	Inquire
SPC-0001-30		30X	Inquire
SWC-0001-10	AccuTarget ™ qPCR Screening Kit-Whole Custom Kit (Modify the plate design into two more pathway)	10X	Inquire
SWC-0001-20		20X	Inquire
SWC-0001-30		30X	Inquire

▶Primer set rearrangment

If you wish to add or modify primer sets, you may use our custom service.
Please send an email to order@bioneer.co.kr with the pathway number and Gene ID/P number, along with the well number that needs to be excluded.
You may search the Gene ID using our Prdesigned siRNA order page.
If you cannot find the gene, please write the details, including the gene name and the ID you wish to include in the email.

Custom Order Service Inquiry: order@bioneer.co.kr
Other Technical / Specifications Inquiry: qPCRarray@bioneer.com

AccuTarget™ qPCR Screening Kit

Overview

Features and Benefits

Applications

Experimental Data

◈ Manual

◈ Flyer

◈ MSDS

◈ Quality Assurance