AccuPower® qPCR Array System: Human Cancer qPCR panel kit (96 well plate)

AccuPower^® qPCR Array System: Human Cancer qPCR panel kit is optimized for GreenStar™. Cancer-related genes are composed of 88 kinds of apoptosis invasion & metastasis, angiogenesis, oncogenes & tumor suppressor, signal transduction & transcription factors, cell cycle & DNA damage repair and 8 kinds of correction genes.

• This product is manufactured as a 96-well plate (Cat. No. 3111-53, Bioneer) compatible with BIONEER's Exicycler™ 96 (Real-Time PCR System).
If you want to make a plate compatible with a third-party equipment, there is a separate cost, so please send an order email to qPCRarray@bioneer.co.kr

• This product only contains pre-dispensed qPCR primer and probe, so a master mix has to be purchased separately.

$280.00

Cat. No.

S-6042-PH1

Product Overview

Features and Benefits

Reliability
Production procedures are in accordance with the MIQE guideline^[1] standard, the qPCR paper criterion proposed by Real-Time PCR experts.

[1] Bustin, S.A., et al. 2009. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments, Clinical Chemistry 55:4, 611-622.
Fidelity
qPCR primer supplied in the kit guarantees qPCR efficiency of 90-110%, and a detection capacity 100 copies per run.
User-friendly Protocol
Simplified procedure allow a head-start, After an addition of the target template and a Master Mix only, the user can start the experiment.
Economical Price
Reduced time and cost to design a primer, synthesieze, and test for efficiency.

Gene Table

Well	Gene symbol	Accession #	Description	Gene name
A1	ABL1	NM_005157, NM_007313	ABL proto-oncogene 1, non-receptor tyrosine kinase	RP11-83J21.1, ABL, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl
B1	AKT1	NM_001014431, NM_001014432, NM_005163	v-akt murine thymoma viral oncogene homolog 1	AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
C1	ALK	NM_004304	Anaplastic lymphoma receptor tyrosine kinase	CD246, NBLST3
D1	ANGPT1	NM_001146, NM_001199859	Angiopoietin 1	AGP1, AGPT, ANG1
E1	ANGPT2	NM_001118887, NM_001118888, NM_001147	Angiopoietin 2	AGPT2, ANG2
F1	APAF1	NM_001160, NM_013229, NM_181861, NM_181868, NM_181869	Apoptotic peptidase activating factor 1	APAF-1, CED4
G1	APC	NM_000038, NM_001127510, NM_001127511	Adenomatous polyposis coli	AI047805, AU020952, AW124434, CC1, Min, mAPC
H1	ATR	NM_001184	ATR serine/threonine kinase	FCTCS, FRP1, MEC1, SCKL, SCKL1
A2	BAD	NM_004322, NM_032989	BCL2-associated agonist of cell death	BBC2, BCL2L8
B2	BAX	NM_001291428, NM_001291429, NM_001291430, NM_001291431, NM_004324, NM_138761, NM_138763, NM_138764	BCL2-associated X protein	BCL2L4
C2	BCL2	NM_000633, NM_000657	B-cell CLL/lymphoma 2	Bcl-2, PPP1R50
D2	BCL2L1	NM_001191, NM_138578	BCL2-like 1	RP5-857M17.3, BCL-XL/S, BCL2L, BCLX, BCLXL, BCLXS, Bcl-X, PPP1R52, bcl-xL, bcl-xS
E2	BCL6	NM_001130845, NM_001134738, NM_001706	B-cell CLL/lymphoma 6	BCL5A, LAZ3, ZBTB27, ZNF51, BCL6
F2	BRAF	NM_004333	B-Raf proto-oncogene, serine/threonine kinase	B-RAF11, NS7, RAFB1, BRAF
G2	BRCA1	NM_007294, NM_007297, NM_007298, NM_007299, NM_007300	Breast cancer 1, early onset	BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
H2	BRCA2	NM_000059	Breast cancer 2, early onset	RP11-298P3.4, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11
A3	CASP8	NM_001080124, NM_001080125, NM_001228, NM_033355, NM_033356, NM_033358	Caspase 8, apoptosis-related cysteine peptidase	ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
B3	CCND1	NM_053056	Cyclin D1	BCL1, D11S287E, PRAD1, U21B31
C3	CCND2	NM_001759	Cyclin D2	KIAK0002, MPPH3
D3	CCND3	NM_001136017, NM_001136125, NM_001136126, NM_001287427, NM_001287434, NM_001760	Cyclin D3	RP5-973N23.3
E3	CDK2	NM_001290230, NM_001798, NM_052827	Cyclin-dependent kinase 2	CDKN2, p33(CDK2)
F3	CDK4	NM_000075	Cyclin-dependent kinase 4	CMM3, PSK-J3
G3	CDK6	NM_001145306, NM_001259	Cyclin-dependent kinase 6	MCPH12, PLSTIRE
H3	CDKN1A	NM_000389, NM_001220777, NM_001220778, NM_001291549, NM_078467	Cyclin-dependent kinase inhibitor 1A (p21, Cip1)	CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
A4	CDKN2A	NM_000077, NM_001195132, NM_058195, NM_058197	Cyclin-dependent kinase inhibitor 2A	ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16
B4	CFLAR	NM_001127183, NM_001127184, NM_001202515, NM_001202516, NM_001202517, NM_001202518, NM_001202519, NM_003879	CASP8 and FADD-like apoptosis regulator	CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS
C4	CHEK2	NM_001005735, NM_001257387, NM_007194, NM_145862	Checkpoint kinase 2	RP11-436C9.1, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
D4	CTNNB1	NM_001098209, NM_001098210, NM_001904	Catenin (cadherin-associated protein), beta 1, 88kDa	OK/SW-cl.35, CTNNB, MRD19, armadillo
E4	ERBB2	NM_001005862, NM_001289936, NM_001289937, NM_001289938, NM_004448	Erb-b2 receptor tyrosine kinase	CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
F4	MECOM	NM_001105077, NM_001105078, NM_001163999, NM_001164000, NM_001205194, NM_004991, NM_005241	MDS1 and EVI1 complex locus	hCG_1640438, AML1-EVI-1, EVI1, MDS1, MDS1-EVI1, PRDM3
G4	EWSR1	NM_001163285, NM_001163286, NM_001163287, NM_005243, NM_013986	EWS RNA-binding protein 1	AC002059.7, EWS, bK984G1.4
H4	FADD	NM_003824	Fas (TNFRSF6)-associated via death domain	GIG3, MORT1
A5	FGFR2	NM_000141, NM_001144913, NM_001144914, NM_001144915, NM_001144916, NM_001144917, NM_001144918, NM_001144919, NM_022970, NM_023029	Fibroblast growth factor receptor 2	BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
B5	FGFR3	NM_000142, NM_001163213, NM_022965	Fibroblast growth factor receptor 3	ACH, CD333, CEK2, HSFGFR3EX, JTK4
C5	FOS	NM_005252	FBJ murine osteosarcoma viral oncogene homolog	AP-1, C-FOS, p55
D5	FOXO1	NM_002015	Forkhead box O1	FKH1, FKHR, FOXO1A
E5	FOXO3	NM_001455, NM_201559	Forkhead box O3	AF6q21, FKHRL1, FKHRL1P2, FOXO2A, FOXO3
F5	GMPS	NM_003875	Guanine monphosphate synthase
G5	HMGA2	NM_001300918, NM_001300919, NM_003483, NM_003484	High mobility group AT-hook 2	BABL, HMGI-C, HMGIC, LIPO, STQTL9
H5	IGF1	NM_000618, NM_001111283, NM_001111284, NM_001111285	Insulin-like growth factor 1	IGF-IA, IGFI, IGF1
A6	JAK2	NM_004972	Janus kinase 2	JTK10, THCYT3
B6	MAP2K1	NM_002755	Mitogen-activated protein kinase kinase 1	CFC3, MAPKK1, MEK1, MKK1, PRKMK1
C6	MDM2	NM_001145337, NM_001145339, NM_001145340, NM_001278462, NM_002392	MDM2 proto-oncogene, E3 ubiquitin protein ligase	ACTFS, HDMX, hdm2
D6	MET	NM_000245, NM_001127500	MET proto-oncogene, receptor tyrosine kinase	AUTS9, HGFR, RCCP2,c-Met
E6	KMT2A	NM_001197104, NM_005933	Lysine (K)-specific methyltransferase 2A	hCG_1732268, ALL-1, CXXC7, HRX, HTRX1, MLL, MLL/GAS7, MLL1, MLL1A, TET1-MLL, TRX1, WDSTS
F6	MMP2	NM_001127891, NM_001302508, NM_001302509, NM_001302510, NM_004530	Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)	CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1
G6	MMP9	NM_004994	Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)	CLG4B, GELB, MANDP2, MMP-9
H6	MTA1	NM_001203258, NM_004689	Metastasis associated 1
A7	MTA2	NM_004739	Metastasis associated 1 family, member 2	MTA1L1, PID
B7	MYC	NM_002467	v-myc avian myelocytomatosis viral oncogene homolog	MRTL, bHLHe39, c-Myc, MYCC
C7	NF1	NM_000267, NM_001042492, NM_001128147	Neurofibromin 1	NFNS, VRNF, WSS
D7	NF2	NM_000268, NM_016418, NM_181825, NM_181828, NM_181829, NM_181830, NM_181831, NM_181832, NM_181833	Neurofibromin 2 (merlin)	ACN, BANF, SCH
E7	NFKB1	NM_001165412, NM_003998	Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1	EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkappaB, p105, p50
F7	NFKBIA	NM_020529	Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha	IKBA, MAD-3, NFKBI
G7	NOTCH1	NM_017617	Notch 1	AOS5, AOVD1, TAN1, hN1
H7	NTRK1	NM_001007792, NM_001012331, NM_002529	Neurotrophic tyrosine kinase, receptor, type 1	MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA
A8	NTRK3	NM_001007156, NM_001012338, NM_001243101, NM_002530	Neurotrophic tyrosine kinase, receptor, type 3	TRKC, gp145(trkC)
B8	PDGFRA	NM_006206	Platelet-derived growth factor receptor, alpha polypeptide	CD140A, PDGFR-2, PDGFR2, RHEPDGFRA
C8	PDGFRB	NM_002609	Platelet-derived growth factor receptor, beta polypeptide	CD140B, IBGC4, IMF1, JTK12, PDGFR, PDGFR-1, PDGFR1
D8	PLAU	NM_001145031, NM_002658	Plasminogen activator, urokinase	RP11-417O11.1, ATF, BDPLT5, QPD, UPA, URK, u-PA
E8	PLAUR	NM_001005376, NM_001005377, NM_001301037, NM_002659	Plasminogen activator, urokinase receptor	CD87, U-PAR, UPAR, URKR
F8	PTEN	NM_000314	Phosphatase and tensin homolog	10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, TEP1, PTEN
G8	RAF1	NM_002880	Raf-1 proto-oncogene, serine/threonine kinase	CMD1NN, CRAF, NS5, Raf-1, c-Raf
H8	RARA	NM_000964, NM_001024809, NM_001145301, NM_001145302	Retinoic acid receptor, alpha	NR1B1, RAR
A9	RB1	NM_000321	Retinoblastoma 1	RP11-174I10.1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110
B9	RET	NM_020630, NM_020975	Ret proto-oncogene	CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC-ELE1, RET51, RET
C9	RUNX1	NM_001001890, NM_001122607, NM_001754	Runt-related transcription factor 1	AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB
D9	SERPINB5	NM_002639	Serpin peptidase inhibitor, clade B (ovalbumin), member 5	PI5, maspin
E9	SERPINE1	NM_000602	Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1	PAI, PAI-1, PAI1, PLANH1
F9	SYK	NM_001135052, NM_001174167, NM_001174168, NM_003177	Spleen tyrosine kinase	p72-Syk
G9	TAL1	NM_001287347, NM_001290403, NM_001290404, NM_001290405, NM_001290406, NM_003189	T-cell acute lymphocytic leukemia 1	SCL, TCL5, bHLHa17, tal-1
H9	TERT	NM_001193376, NM_198253	Telomerase reverse transcriptase	CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT
A10	TFE3	NM_001282142, NM_006521	Transcription factor binding to IGHM enhancer 3	RCCP2, RCCX1, TFEA, bHLHe33
B10	TFEB	NM_001167827, NM_001271943, NM_001271944, NM_001271945, NM_007162	Transcription factor EB	RP4-696P19.3, ALPHATFEB, BHLHE35, TCFEB
C10	TGFB1	NM_000660	Transforming growth factor, beta 1	CED, DPD1, LAP, TGFB, TGFbeta
D10	TGFBR1	NM_001130916, NM_004612	Transforming growth factor, beta receptor 1	RP11-96L7.1, AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TGFR-1, tbetaR-I
E10	THBS1	NM_003246	Thrombospondin 1	THBS, THBS-1, TSP, TSP-1, TSP1
F10	TIMP1	NM_003254	TIMP metallopeptidase inhibitor 1	RP1-230G1.3, CLGI, EPA, EPO, HCI, TIMP
G10	TIMP2	NM_003255	TIMP metallopeptidase inhibitor 2	CSC-21K, DDC8
H10	TLX1	NM_001195517, NM_005521	T-cell leukemia homeobox 1	HOX11, TCL3
A11	TNF	NM_000594	Tumor necrosis factor	DADB-70P7.1, DIF-alpha, TNFA, TNFSF2, TNF
B11	TNFRSF10B	NM_003842, NM_147187	Tumor necrosis factor receptor superfamily, member 10b	UNQ160/PRO186, CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9
C11	TNFRSF1A	NM_001065	Tumor necrosis factor receptor superfamily, member 1A	CD120a, FPF, MS5, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2, TNFR55, TNFR60, p55, p55-R, p60
D11	TNFRSF25	NM_001039664, NM_003790, NM_148965, NM_148966, NM_148967, NM_148970	Tumor necrosis factor receptor superfamily, member 25	RP4-650H14.2, APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR
E11	TP53	NM_000546, NM_001126112, NM_001126113, NM_001126114, NM_001126115, NM_001126116, NM_001126117, NM_001126118, NM_001276695, NM_001276696, NM_001276697, NM_001276698, NM_001276699, NM_001276760, NM_001276761	Tumor protein p53	BCC7, LFS1, P53, TRP53
F11	TRADD	NM_003789	TNFRSF1A-associated via death domain	Hs.89862
G11	VEGFA	NM_001025366, NM_001025367, NM_001025368, NM_001025369, NM_001025370, NM_001033756, NM_001171622, NM_001171623, NM_001171624, NM_001171625, NM_001171626, NM_001171627, NM_001171628, NM_001171629, NM_001171630, NM_001204384, NM_001204385, NM_001287044, NM_003376	Vascular endothelial growth factor A	RP1-261G23.1, MVCD1, VEGF, VPF
H11	WT1	NM_000378, NM_001198551, NM_001198552, NM_024424, NM_024426	Wilms tumor 1	AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33

Technical/Specs

▶ Components

It consists of 88 kinds of cancer-related genes, 5 kinds of reference genes, and 3 types of control. Cancer-related genes were screened in the following five Human cancer libraries.

Apoptosis invasion & metastasis
Angiogenesis
Oncogenes & tumor suppressor
Signal transduction & transcription factors
Cell cycle & DNA damage repair

▶ Reference Genes and qPCR Controls

• Reference genes (internal control)

Provides five genes widely used for internal control during the relative quantitative analysis

• Genomic DNA Control (GDC)

Provides a genomic DNA control to detect gDNA contamination of samples

• No Template Control (NTC)

Provides no template control to check for contamination during the experiment

• Positive PCR Control (PPC)

Provides positive PCR control to test qPCR amplification efficiency with prepared template and primer

Literature/Support

◈ Manual

Quick manual_AccuPower^® qPCR Array System: Human Cancer qPCR panel kit

User guide_AccuPower^® qPCR Array System: Human Cancer qPCR panel kit

◈ MSDS

MSDS_AccuPower® qPCR Array System Human Cancer qPCR panel kit EN ver.2.pdf

Ordering Info

1Service Price

Cat. No.	Product Description	Gene	Price
S-6042-PH1	AccuPower^® qPCR Array System: Human Cancer qPCR panel kit	88	$S-6042-PH1
S-6042-S200	* AccuPower^® qPCR Array System: Single gene qPCR primer	1	Inquire

* You can purchase 200 rxn of desired genes among the constitutive genes of cancer.

2How to request service

• This product is manufactured as a 96 well plate (Cat. No. 3111 53, Bioneer) compatible with Exicycler™ 96 (Real Time PCR System, Bioneer).
• If you want to make a plate compatible with a third-party equipment, please send an order email to qPCRarray@bioneer.co.kr

3Consultation

• Cosultation hours: Weekdays from 9:00 am ~ 6:00 pm (closed on weekends and holudays) (GMT +9)
• Telephone: +82-42-930-8673 (Gene Expression Analysis Team)
• E-mail: qPCRarray@bioneer.co.kr