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mRNA Synthesis Custom Order
After the successful entry of the COVID-19 mRNA vaccine into the market, active research is underway for the development of mRNA vaccines and therapeutics. BIONEER provides custom mRNA development process through extensive research optimizing in vitro transcription (IVT) and purification for stable and efficient mRNA synthesis. BIONEER mRNA synthesis service offers a variety of options to provide tailored mRNA solutions to suit your needs.
Overview
BIONEER provides various types of mRNA using our advanced mRNA synthesis technology. For mRNA synthesis, you can either provide the DNA template or it can be linked with our in-house Gene Synthesis Service.
Our mRNA Synthesis Service is categorized into two options: Standard mRNA Synthesis Service and Complete mRNA Synthesis Service. The standard service provides only the RNA coding sequence, with optional choices of 5' cap and 3' poly (A) tail. Complete service offers not only the RNA coding sequences but also includes 5' cap, 3' poly (A) tail, and 5' & 3' UTR. Depending on your experimental designs, you may select the service that suits your needs.
RNA coding sequence | 5' cap | 3' poly (A) tail | 5' & 3' UTR | |
Standard mRNA Synthesis | O | Option | Option | X |
Complete mRNA Synthesis | O | O | O | O |
The ordered mRNA is shipped in a lyophilized (freeze-dried) form, along with gel electrophoresis and NanoDrop absorbance data.
Features and Benefits
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Customize your desired mRNA with 5' or 3' poly (A) tailing modification options according to your experimental designs.
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Microgram to milligram scale mRNA production
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Modified rNTPs to suit your application needs
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Premade reporter gene (eGFP, mCherry, Luciferase) mRNA is available at reasonable prices.
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RNA-Lipid nanoparticle (LNP) service is available.
Experimental Data
Ctrl | 3 months | 6 months | 12 months | |
Amount(μg) | 11.3 | 11.4 | 11.2 | 11.4 |
A260/A280 | 1.98 | 2.00 | 1.99 | 1.99 |
A260/A230 | 2.44 | 2.41 | 2.43 | 2.45 |
Negative control : Uncapped mRNA, Positive control : CleanCap (Trilink) mRNA,
Bioneer’s VCE : Cap-0 and Cap-1 mRNAs capped by Bioneer’s vaccinia capping enzyme