rA(Bz)-CE phosphoramidite

• When synthesizing oligonucleotides, It is recommended to treat with 3 hours at 40°C AMA reagent(ammonia+methylamine gas) or use methylamine gas for cleavage and deprotection.

• Cleavage and deprotection procedures for RNA synthesis are similar to DNA synthesis, with an additional step to remove the 2'-OH protecting group.

• The 2'-OH group is protected by a tert-butyldimethylsilyl(TBDMS) group to prevent derivatization and degradation during the synthesis cycle.

• Synthesizing RNA oligonucleotides has similar process with synthesizing DNA oligonucleotides, but requires additional processes to remove 2'-OH protecting group.

• Our company makes sure that the supplied nucleoside phosphoramidites retain purities greater than 99% with our strict standards by using 31P-NMR and RP-HPLC.

• All reagents are conveniently packaged for ABI instruments. Expedite- and Mermade-compatible vials are also available upon request.

Figure 1. HPLC data : ≥ 99%	Figure 2. 31P-NMR data : ≥ 99%