AccuPower® Bacteroides spp. Real-time PCR Kit

AccuPower^® Bacteroides spp. Real-Time PCR Kit is a product that can specifically detect Bacteroides spp. by real-time PCR. Bacteroides spp. can cause peritonitis or inflammatory bowel disease.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.

※ This product is shipped in dry ice.

$1,000.00

Cat. No.

K-6875

Product Overview

Overview

Bacteroides spp. are gram-negative bacteria that make up a part of the intestinal flora and can cause peritonitis when infected with a wound in the intestinal tissue or trigger bacteremia by entering the bloodstream. It is also known to cause inflammatory bowel diseases such as Crohn's disease and ulcerative colitis. In addition, it is a pathogen that requires attention because substances produced during metabolism contribute to the growth of other pathogens.

Features and Benefits

Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

Technical/Specs

▶ Specification

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
DEPC-D.W.	1.8 ml
Positive Control (1x10⁸ copies/μl)	50 μl

▶ Experimental Data

Figure 1. Sensitivity of Bacteroides spp. (B. caccae) detection experiment result.

Figure 2. Sensitivity of Bacteroides spp. (B. fragilis) detection experiment result.

Figure 3. Sensitivity of Bacteroides spp. (B. thetaiotaomicron) detection experiment result.

AccuPower^® Bacteroides spp. Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).

Literature/Support