AccuPower® Bacteroides fragilis Real-time PCR Kit

AccuPower^® Bacteroides fragilis Real-Time PCR Kit is a product that can specifically detect Bacteroides fragilis (B. fragilis) by real-time PCR. B. fragilis is a gram-negative bacterium known to cause peritonitis.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

$1,000.00

Cat. No.

K-6859

Product Overview

Overview

B. fragilis is a gram-negative bacterium known to cause primarily peritonitis and bacteremia. B. fragilis is a normal flora that is found in the intestines of the human body, especially in the colon. The toxins produced by B. fragilis can cause inflammatory diarrhea and inflammatory bowel disease. It can also cause intra-abdominal infections due to wounds to the intestinal tissue barrier or bacteremia due to inflow into the bloodstream.

Features and Benefits

Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

Technical/Specs

▶ Specification

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
DEPC-D.W.	1.8 ml
Positive Control (1x10⁸ copies/μl)	50 μl

▶ Experimental Data

Figure 1. Sensitivity of Bacteroides fragilis detection experiment result.
AccuPower^® Bacteroides fragilis Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).

Literature/Support