AccuPower® Shigella sonnei Real-time PCR Kit

AccuPower^® Shigella sonnei Real-Time PCR Kit is a product that can specifically detect Shigella sonnei (S. sonnei) by real-time PCR. S. sonnei is a gram-negative bacterium known to cause dysentery.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

$1,000.00

Cat. No.

K-6854

Product Overview

Overview

S. sonnei is a gram-negative bacterium known to mainly cause dysentery, sepsis and meningitis. S. sonnei is known as a representative pathogen causing Shigellosis along with S. boydii, S. flexneri and S. dysenteriae. Shigellosis occurs by having food contaminated with pathogens. Since mass infections are commonly reported in unsanitary places, careful personal hygiene management can prevent infection caused by S. sonnei.

Features and Benefits

Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

Technical/Specs

▶ Specification

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
DEPC-D.W.	1.8 ml
Positive Control (1x10⁸ copies/μl)	50 μl

▶ Experimental Data

Figure 1. Sensitivity of Shigella sonnei detection experiment result.
AccuPower^® Shigella sonnei Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).

Literature/Support