Overview
S. sobrinus is a gram-positive bacterium that causes tooth decay, periodontitis or endocarditis. S. sobrinus, along with S. mutans, is a representative bacterium that causes a cavity. In most cases, its transmitted vertically from the mother and actively reproduces when consuming excessive sugar during infancy or growing period.
Features and Benefits
- Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
- Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.
▶ Specification
| Components |
Amount |
| 2X Master Mix |
625 μl x 2 ea |
| Oligo Mix |
500 μl |
| DEPC-D.W. |
1.8 ml |
| Positive Control (1x108 copies/μl) |
50 μl |
▶ Experimental Data
Figure 1. Sensitivity of Streptococcus sobrinus detection experiment result.
AccuPower® Streptococcus sobrinus Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).
