AccuPower® Klebsiella pneumoniae Real-Time PCR Kit

AccuPower^® Streptococcus pasteurianus Real-Time PCR Kit is a product that can specifically detect Streptococcus gallolyticus subsp. pasteurianus (S. pasteurianus) by real-time PCR. S. pasteurianus is a gram-positive bacterium that causes sepsis.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

$1,000.00

Cat. No.

K-6821

Product Overview

Overview

K. pneumoniae is a gram-negative bacterium that causes pneumonia, urinary tract infections, and sepsis. K. pneumoniae is an opportunistic pathogen that is usually present in the mouth, skin, and intestines of a healthy person, but when inhaled or flowed into the lungs or bloodstream through a catheter, it is pathogenic. K. pneumoniae causes various diseases depending on the infected area. Especially, pneumonia commonly occurs in patients with reduced immunity such as alcoholics and diabetic patients.

Features and Benefits

Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

Technical/Specs

▶ Specification

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
DEPC-D.W.	1.8 ml
Positive Control (1x10⁸ copies/μl)	50 μl

▶ Experimental Data

Figure 1. Sensitivity of Klebsiella pneumoniae detection experiment result.

AccuPower^® Klebsiella pneumoniae Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).

Figure 2. Limit of Detection (LoD) of AccuPower® Klebsiella pneumoniae Real-Time PCR Kit

AccuPower^® Klebsiella pneumoniae Real-Time PCR Kit was used, and 24 replicates were performed for each section (1000, 333, 111, 37, 12.3, 4.1, 1.4 copies/rxn), and the measured results were analyzed through probit analysis. Through statistical analysis, the lowest concentration detectable by more than 95% was established as LoD.
When the cut-off value was calculated using the LoD test results, it was confirmed to be 41.74 Ct.

Literature/Support