AccuPower® Staphylococcus epidermidis Real-Time PCR Kit

AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit is a product that can specifically detect Staphylococcus epidermidis (S. epidermidis) by real-time PCR. S. epidermidis is a gram-positive bacterium, one of 40 species belonging to Staphylococcus. Patients with compromised immune systems are at risk of infection caused by S. epidermidis.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

$1,000.00

Cat. No.

K-6817

Product Overview

Overview

S. epidermidis infection can be a risk for patients using surgical implants due to defective heart valves. When the infection occurs, a biofilm is formed on the implanted device. Antibiotics are rarely effective at removing the biofilm formed by S. epidermidis, and the most common method of treatment is removal or replacement of the infected device.

Features and Benefits

Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

Technical/Specs

▶ Specification

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
DEPC-D.W.	1.8 ml
Positive Control (1X10⁸ copies/μl)	50 μl

▶ Experimental Data

Figure 1. Sensitivity of Staphylococcus epidermidis detection experiment result.

AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).

Figure 2. Limit of Detection (LoD) of AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit

AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit was used, and 24 replicates were performed for each section (1000, 333, 111, 37, 12.3, 4.1, 1.4 copies/rxn), and the measured results were analyzed through probit analysis. Through statistical analysis, the lowest concentration detectable by more than 95% was established as LoD.
When the cut-off value was calculated using the LoD test results, it was confirmed to be 41.19 Ct.

Literature/Support

◈ Manual

• Manual_AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit

◈ MSDS

• MSDS_K-6817 AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit EN ver.0

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

Cat.No.	Product Description	Option
K-6817	AccuPower^® Staphylococcus epidermidis Real-Time PCR Kit	1.25 ml of 2X Master Mix solution / 100 tests