AccuPower® Borrelia burgdorferi Real-Time PCR Kit

AccuPower^® Borrelia burgdorferi Real-Time PCR Kit is a product that can specifically detect Borrelia burgdorferi (B. burgdorferi) by real-time PCR. B. burgdorferi is causative agents of Lyme disease.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

$1,000.00

Cat. No.

K-6803

Product Overview

Overview

B. burgdorferi is a gram-negative bacterium known as one of the causative agents of Lyme disease. B. burgdorferi hosts black-legged ticks and is transmitted to human. Lyme disease characteristically causes erythema migraines, and also fever, headache, and fatigue. Lyme disease is diagnosed by in three stages, and if it is not treated in the first stage, the infection spreads through the blood to whole body organs causing arrhythmias, myocarditis, and encephalitis.

Features and Benefits

Convenience
All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

Technical/Specs

▶ Specification

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
DEPC-D.W.	1.8 ml
Positive Control (1X10⁸ copies/μl)	50 μl

▶ Experimental Data

Figure 1. Sensitivity of Borrelia burgdorferi detection experiment result.

AccuPower^® Borrelia burgdorferi Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-time Quantitative Thermal cycler (Bioneer Co.).

Figure 2. Limit of Detection (LoD) of AccuPower^® Borrelia burgdorferi Real-Time PCR Kit

AccuPower^® Borrelia burgdorferi Real-Time PCR Kit was used, and 24 replicates were performed for each section (1000, 333, 111, 37, 12.3, 4.1, 1.4 copies/rxn), and the measured results were analyzed through probit analysis. Through statistical analysis, the lowest concentration detectable by more than 95% was established as LoD.
When the cut-off value was calculated using the LoD test results, it was confirmed to be 40.06 Ct.

Literature/Support