AccuPower® GreenStar™ qPCR PreMix uses dsDNA binding dye in real-time PCR to accurately quantify the target gene samples from various kinds of samples quickly and accurately. It is applied with Bioneer’s patent enzyme-mediated HotStart technology to enhance reaction specificity and PCR amplification efficiency. Furthermore, all the essential components are vacuum dried in each tube to prevent cross-contamination.
※ This product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
This product is applied with PyroHotStart technology, Bioneer’s patent technology, to minimize non-specific reactions by utilizing pyrophosphate and pyrophosphatase enzymes during the zero cycle, increase PCR reaction efficiency through eliminating PCR inhibitor PPi, making it capable to amplify even a trace amount of template DNA.
(a) Inhibition of PCR reaction in the zero cycle
After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.
Also, since DNA polymerases requires Mg ions to activate, PPi, having high affinities with Mg2+ ions, traps them to inhibit the PCR.
(b) Denaturation
PPase included in the mixture is activated when the temperature rises during the denaturation step to decompose PPi into Pi for dissociating the Mg2+ ions.
The isolated ions bind with DNA polymerases to activate, allowing the PCR reaction to proceed.
(c) Annealing & Extension
The activated DNA polymerases amplifies the target DNA as the PCR cycle progresses.
Wide choice of real-time PCR instruments for optimized results
Simplified products as all reactants essential for real-time PCR included in each tubes to readily start by adding template DNA, probe & primer for target gene, and D.W.
Enhanced stability having more resistance to degradation than the solution type products with a stabilizer included in the Real-Time PCR reaction mixture
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables with mass production under ISO 9001 quality system
• Real-Time Quantification of DNA/cDNA targets.
• Quantification of gene expression
• Microbial/Viral Pathogen Detection.
Specificity activity
Figure. Real-Time PCR Data of AccuPower® Greenstar™ qPCR PreMix
AccuPower® Greenstar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (10 fg ~ 10 ng/reaction). All data were obtained using Exicycler™ 96 Real-time Quantitative Thermal Block.
(A) : Amplification curve. Lambda DNA primers were added into AccuPower® Greenstar™ qPCR PreMix. A series of Lambda DNA positive control diluents were tested.
(B) : Standard curve. Data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
(C) : Melting curve analysis of AccuPower® Greenstar™ qPCR PreMix. - The melting curve shows that only single amplified PCR product was obtained in all template range.
