AccuPower® Dualstar™ qPCR PreMix enables accurate and rapid quantification of target genes in a wide range of samples through real-time PCR with Hydrolysis probe method. By applying Bioneer's patented enzyme-mediated HotStart technology, reaction specificity and PCR amplification efficiency have been improved, with more convienance and accuracy, having each tube packaged with premixed vacuum dried reagents essential for real-time PCR to prevent cross-contamination through the repetitive use of master mix products.
※This product is shipped in dry ice.
(a) Inhibition of PCR reaction in the zero cycle
After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.
Also, since DNA polymerases requires Mg ions to activate, PPi, having high affinities with Mg2+ ions, traps them to inhibit the PCR.
(b) Denaturation
PPase included in the mixture is activated when the temperature rises during the denaturation step to decompose PPi into Pi for dissociating the Mg ions.
The isolated ions bind with DNA polymerases to activate, allowing the PCR reaction to proceed.
(c) Annealing & Extension
The activated DNA polymerases amplifies the target DNA as the PCR cycle progresses.
Minimized non-specific amplification by applying PyroHotStart technology, a Bioneer’s proprietary patented technology that uses of pyrophosphate and pyrophosphatase enzymes to reduce non-specific reactions during zero cycles & Maximized PCR reaction efficiency through elimination of PCR inhibitor PPi in every amplification cycle, allowing amplification of template DNA present even in a trace amount
Compatibility
Wide choice of real-time PCR instruments for optimized results
Effective quantitative PCR results from a gene regardless of its type, including DNA, cDNA and high GC template.
Simplified procedure with all reactants essential for real-time PCR included in each tubes packaged with sufficient amounts for 1 run to readily start by adding template DNA, probe & primer for target gene, and D.W.
Enhanced stability allowing enzyme activity to stay stable for up to 2 years at -20 ° C by including a stabilizer to the PCR reaction mixture, .
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables by mass production under ISO 9001 quality system
• Gene expression profiling
• Target DNA quantification
• Microbial detection
• Viral/bacterial pathogen load determination
• Probe-based real-time PCR primer pair performance evaluation
Figure. Real-Time PCR Data of AccuPower® Dualstar™ qPCR PreMix
AccuPower® Dualstar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (107 ~ 101 copies/reaction).
(A) : Amplification curve. West Nile Virus (WNV) primers and TaqMan-based probe were added into AccuPower® Dualstar™ qPCR PreMix.
A series of WNV positive control diluents were tested.
(B) : Standard curve. All data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
