AccuPower® PRV Master Mix / Real-Time PCR Kit

AccuPower^® PRV Master Mix/Real-Time PCR Kit is a product which can detect Piscine orthoreovirus, causes the disease heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon, by real-time PCR.

※ Note: For research use only. Not for use in diagnostic procedures.
※ This product is shipped in dry ice.

As low as $350.00

Cat. No.

Product Overview

Overview

Piscine orthoreovirus (PRV) is a serious virus in salmon aquaculture belonging to the family Reoviridae. PRV has been found present at higher concentration in fish with various diseases. This disease includes HSMI, jaundice syndrome, proliferative darkening syndrome and erythrocytic body inclusion syndrome. PRV is thought to mainly affect aquacultured and maricultured fish stocks.

Features

Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template RNA, oligo mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template RNA

Technical/Specs

▶ Specification

K-2992

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
Positive Control (1x10⁸ copies/μl)	50 μl
DEPC-D.W.	1.3 ml

K-2993

Components	Amount
PreMix	8-strips x 12 ea
Positive Control (1x10⁸ copies/μ)	50 μl
Sealing film	1 ea
DEPC-D.W.	1.3 ml

▶ Experimental data

Figure 1. Summary of result using AccuPower® PRV Master Mix/Real-Time PCR Kit
This data was obtained by using AccuPower^® PRV Master Mix/Real-Time PCR Kit and Exicycler™ 96 Real-time Quantitative Thermal Block (Bioneer Co.).

Literature/Support

◈ Manual

• Manual_AccuPower^® PRV Master Mix

• Manual_AccuPower^® PRV Real-Time PCR Kit

◈ MSDS

• MSDS_AccuPower^® PRV Master Mix

• MSDS_AccuPower^® PRV Real-Time PCR Kit

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

Cat. No.	Product Description	Option
K-2992	AccuPower^® PRV Master Mix	1.25 ml of 2X Master Mix solution, 100 tests
K-2993	AccuPower^® PRV Real-Time PCR Kit	Exicycler™ 8-well strips / 96 tubes