AccuPower® Salmon Disease Multiplex Master Mix / Real-Time PCR Kit

AccuPower^® Salmon Disease Multiplex Master Mix/Real-Time PCR Kit is a product that can simultaneously detect diseases infecting salmon (Infectious Pancreatic Necrosis (IPN), Infectious Salmon Anaemia (ISA), Bacterial Kidney Disease (BKD), Salmonid Rickettsial Septicaemia (SRS)) through real-time PCR.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

As low as $500.00

Cat. No.

Product Overview

Overview

IPN, ISA, BKD, SRS are caused by Infectious Pancreatic Necrosis Virus (IPNV), Infectious Salmon Anaemia Virus (ISAV), Renibacterium salmoninarum, Piscirickettsia salmonis. Clinical signs of these salmon diseases include swollen abdomen or eyes or darkening of the skin, pale gills, haemorrhage at the base of the fins, lesions of the skin and death in acute cases. These infectious diseases are causing and increasing huge economic losses in salmon farming industry especially Chile, as well as in Canada, Ireland, Scotland and Norway.

Features

Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template DNA/RNA, oligo mix, and DEPC-D.W.
Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA/RNA

Technical/Specs

▶ Specification

K-2990

Components	Amount
2X Master Mix	625 μl x 2 ea
Oligo Mix	500 μl
Positive Control (1x10⁸ copies/μl)	50 μl
DEPC-D.W.	1.3 ml

K-2991

Components	Amount
PreMix	8-strips x 12 ea
Positive Control (1x10⁸ copies/μ)	50 μl
Sealing film	1 ea
DEPC-D.W.	1.3 ml

▶ Experimental data

Figure 1. Summary of result using AccuPower® Salmon Disease Multiplex Master Mix/Real-Time PCR Kit

(A) Multi IPNV - FAM, (B) Multi ISAV – Cy5, (C) Multi BKD – Texas RED, (D) Multi SRS - TET, (E) Salmon Disease multiplex
This data was obtained by using AccuPower^® Salmon Disease Multiplex Master Mix/Real-Time PCR Kit and Exicycler™ 96 Real-time Quantitative Thermal Block (Bioneer Co.).

Literature/Support

◈ Manual

• Manual_AccuPower^® Salmon Disease Multiplex Master Mix

• Manual_AccuPower^® Salmon Disease Multiplex Real-Time PCR Kit

◈ MSDS

• MSDS_AccuPower^® Salmon Disease Multiplex Master Mix

• MSDS_AccuPower^® Salmon Disease Multiplex Real-Time PCR Kit

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

Cat. No.	Product Description	Option
K-2990	AccuPower^® Salmon Disease Multiplex Master Mix	1.25 ml of 2X Master Mix solution, 100 tests
K-2991	AccuPower^® Salmon Disease Multiplex Real-Time PCR Kit	Exicycler™ 8-well strips / 96 tubes