Overview
G. Parasuis is a gram-negative bacterium known as one of the pathogens that cause the respiratory infection and multiple tentitis of pigs. G. Parasuis, opportunistic bacteria, infects pigs with weakened immunity and may have symptoms such as pneumonia, cramps, vitality, arthritis, etc. during infection.
Features and Benefits
- Convenience
All reactants necessary for Real-Time PCR are included in a tube, it allows any user to simply perform reaction by adding template DNA and DEPC-D.W.
- Sensitivity
By applying the patented PyroHotStart(Enzyme-mediated HotStart) technology that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.
▶ Specification
| Components |
Amount |
| 2X Master Mix |
625 μl x 2 ea |
| Oligo Mix |
500 μl |
| DEPC-D.W. |
1.8 ml |
| Positive Control (1X108 copies/μl) |
50 μl |
▶ Experimental Data
Figure 1. Glaesserella parasuis detection expriment result
AccuPower® Glaesserella parasuis Real-Time PCR Kit was used. This data was obtained using Exicycler™ 96 Real-Time Quantitative Thermal cycler (Bioneer Co.).
