Overview
Infectious myonecrosis virus (IMNV), the causative agent of infectious myonecrosis, mainly infects shrimp and its clinical signs include malnutrition and partial hardening, leading to death. IMN is causing enormous economic losses to shrimp aquaculture in Vietnam, Malaysia, Thailand, as well as many other Asian countries, and is an aquatic disease designated by the World Organization for Animal Health (WOAH).
Features
- Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template RNA, oligo mix, and DEPC-D.W.
- Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template RNA
▶ Specification
| Components |
Amount |
| Master Mix |
1.5 ml |
| Oligo Mix |
400 μl |
| IMNV Positive Control (2x107 copies/μl) |
50 μl |
| Internal Positive Control (1x105 copies/μl) |
100 μl |
| PC Dilution buffer |
1 ml |
| DEPC-D.W. |
1.3 ml |
▶ Experimental data
Figure 1. Sensitivity of myonecrosis virus (IMNV) detection experiment result
This data was obtained by using AccuPower® IMNV Master Mix and Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
◈ Manual
• Manual_AccuPower® IMNV Master Mix
◈ MSDS
• MSDS_AccuPower® IMNV Master Mix
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
◈ Primer reference
• Tang K.F.J., Wang J. & Lightner D.V. (2004). Quantitation of Taura syndrome virus by real-time RT-PCR with a TaqMan assay. J. Virol. Methods, 115, 109–114.
