Overview
Red sea bream iridoviral disease (RSIVD) causes mass mortality in more than 30 species of fish, mainly sea bream, amberjack, mackerel, etc. Clinical symptoms of RSIVD include lethargy, severe anemia, gill petechiae, and spleen enlargement. RSIVD occurs consistently in East and South-East Asian countries. It is designated as an aquatic disease by the World Organization for Animal Health (WOAH).
Features
- Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template DNA, oligo mix, and DEPC-D.W.
- Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA
▶ Specification
| Components |
Amount |
| Master Mix |
1.5 ml |
| Oligo Mix |
400 μl |
| RSIV/ISKNV Positive Control (2x107 copies/μl) |
50 μl |
| Internal Positive Control (1x105 copies/μl) |
100 μl |
| PC Dilution buffer |
1 ml |
| DEPC-D.W. |
1.3 ml |
▶ Experimental data
Figure 1. Sensitivity of red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV) detection experiment result
This data was obtained by using AccuPower® RSIV/ISKNV Multiplex Master Mix and Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
◈ Manual
• Manual_AccuPower® RSIV/ISKNV Master Mix
◈ MSDS
• MSDS_AccuPower® RSIV/ISKNV Master Mix
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
