Overview
Spring viremia of carp virus (SVCV), the causative agent of spring viremia of carp (SVC), mainly infects carp and has a high mortality rate upon infection. SVC incurs serious economic losses not only in Korea but also in many countries in Europe (United Kingdom, Austria, France, Germany) and North America. It is an aquatic disease designated by the World Organization for Animal Health (WOAH).
Features
- Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template RNA, oligo mix, and DEPC-D.W.
- Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template RNA
▶ Specification
| Components |
Amount |
| Master Mix |
1.5 ml |
| Oligo Mix |
400 μl |
| SVCV Positive Control (2x107 copies/μl) |
50 μl |
| Internal Positive Control (1x105 copies/μl) |
100 μl |
| PC Dilution buffer |
1 ml |
| DEPC-D.W. |
1.3 ml |
▶ Experimental data
Figure 1. Sensitivity of spring viremia of carp virus (SVCV) detection experiment result
This data was obtained by using AccuPower® SVCV Master Mix and Exicycler™ 96 Real-time Quantitative Thermal Block (Bioneer Co.).
◈ Manual
• Manual_AccuPower® SVCV Master Mix
◈ MSDS
• MSDS_AccuPower® SVCV Master Mix
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
