Overview
Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome (EUS), mainly causes clinical symptoms such as darkening of the body, red spots or small-to-large ulcerative lesions on the body surface in freshwater fish. EUS occurs throughout the world, including Asia, Australia, North America, and South Africa. It is an aquatic disease designated by the World Organization for Animal Health (WOAH).
Features
- Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template DNA, oligo mix, and DEPC-D.W.
- Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.
▶ Specification
| Components |
Amount |
| Master Mix |
1.5 ml |
| Oligo Mix |
400 μl |
| EUS Positive Control (2x107 copies/μl) |
50 μl |
| Internal Positive Control (1x105 copies/μl) |
100 μl |
| PC Dilution buffer |
1 ml |
| DEPC-D.W. |
1.3 ml |
▶ Experimental data
Figure 1. Sensitivity of EUS (Aphanomyces invadans) detection experiment result
This data was obtained by using AccuPower® EUS Master Mix and Exicycler™ 96 Real-time Quantitative Thermal Block (Bioneer Co.).
◈ Manual
• Manual_AccuPower® EUS Master Mix
◈ MSDS
• MSDS_AccuPower® EUS Master Mix
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
