Overview
White spot syndrome virus (WSSV), the cause of white spot disease (WSD), mainly infects shrimp, with clinical signs including white spots on the exoskeleton and death due to lack of nutrition. WSD is incurring enormous economic losses to shrimp farming in Vietnam, Malaysia, Thailand, as well as many other Asian countries, and is an aquatic disease designated by the World Organization for Animal Health (WOAH).
Features
- Convenience
All necessary reactants for RT-qPCR such as RocketScript™ RTase, HotStart Taq DNA polymerase are included in a tube allowing the users to perform reaction simply by adding template DNA, oligo mix, and DEPC-D.W.
- Sensitivity
By using BIONEER’s PyroHotStart RT reaction and HotStart Taq DNA Polymerase that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA
▶ Specification
| Components |
Amount |
| Master Mix |
1.5 ml |
| Oligo Mix |
400 μl |
| WSSV Positive Control (2x107 copies/μl) |
50 μl |
| Internal Positive Control (1x105 copies/μl) |
100 μl |
| PC Dilution buffer |
1 ml |
| DEPC-D.W. |
1.3 ml |
▶ Experimental data
Figure 1. Sensitivity of White spot syndrome virus (WSSV) detection experiment result
This data was obtained by using AccuPower® WSSV Master Mix and Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
◈ Manual
• Manual_AccuPower® WSSV Master Mix
◈ MSDS
• MSDS_AccuPower® WSSV Master Mix
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
◈ Primer Reference
• Durand S.V. & Lightner D.V. (2002). Quantitative real time PCR for the measurement of white spot syndrome virus in shrimp. J. Fish Dis., 25, 381–389.
