AccuPower® E. coli EAE PCR kit

AccuPower^®EAE PCR Kit is a ready-to-use premix for PCR that can be used to detect E. coli in livestock by specifically amplifying the E. coli attachment effacement (EAE) gene.

※ Note: For research use only. Not for use in diagnostic or therapeutic procedures.
※ This product is shipped in dry ice.

$370.00

Cat. No.

K-2905

Product Overview

Overview

E. coli enters the organs of animals and causes lesions. It is also a source of many infections such as acute urinary tract infections, peritonitis, hepatitis, sepsis, and meningitis. E. coli can be classified into various categories: enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli (EHEC), etc. Among those, intimin, the product of EAE gene, causes EPEC adhesion to intestinal epithelial cells, which may develop into microvilli atrophy, polar actin, etc. Furthermore, intimin protein can also induce pathogenic characteristics to EHEC and EPEC by inducing bacterial adhesion, which develops into A/E lesions.

Features and Benefits

Convenience & Reproducibility
All reactants necessary for PCR including primers are lyophilized in each PCR tube, providing reproducible results in a convenient way.
Sensitivity
By applying the patented PyroHotStart(Enzyme-mediated HotStart) technology that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.
Stability
Included stabilizer in the PCR reaction mixture provides increased stability compared to solution-type products.

Technical/Specs

▶ Specifications

Type	PreMix
Target	E. coli attachment effacement (EAE)
Instrument	Thermal Cycler
Specimen	Environmental sample etc.
Tests per kit	96 tests

▶ Experimental Data

Figure 1. Sensitivity of EAE detection experiment result.
AccuPower^® EAE PCR Kit was used, and 5 µl of PCR reaction product was electrophoresed on 1.5% TBE Agarose gel.

Literature/Support