AccuPower® E. coli EAE PCR kit

AccuPower® EAE PCR Kit is a ready-to-use premix for PCR that can be used to detect E. coli in livestock by specifically amplifying the E. coli attachment effacement (EAE) gene.

This kit is for research use only and must not be used for diagnostic purposes.

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Cat. No.
K-2905

Overview

E. coli enters the organs of animals and causes lesions. It is also a source of many infections such as acute urinary tract infections, peritonitis, hepatitis, sepsis, and meningitis. E. coli can be classified into various categories: enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli (EHEC), etc. Among those, intimin, the product of EAE gene, causes EPEC adhesion to intestinal epithelial cells, which may develop into microvilli atrophy, polar actin, etc. Furthermore, intimin protein can also induce pathogenic characteristics to EHEC and EPEC by inducing bacterial adhesion, which develops into A/E lesions.

Features and Benefits

  • Convenience & Reproducibility
    All reactants necessary for PCR including primers are lyophilized in each PCR tube, providing reproducible results in a convenient way.

  • Sensitivity
    By applying the patented PyroHotStart(Enzyme-mediated HotStart) technology that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.

  • Stability
    Included stabilizer in the PCR reaction mixture provides increased stability compared to solution-type products.

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