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AccuPower® RT-PCR Master Mix (2X, 1ml)
![](https://eng.bioneer.com/media/catalog/product/cache/image/210x140/af097278c5db4767b0fe9bb92fe21690/_/k/_k-2264__1.jpg)
AccuPower® RT-PCR Master Mix is the Master Mix type of AccuPower® RT-PCR Premix products.
※ Master Mix product is shipped in dry ice
Features and Benefits
- Easy-of-use
Simplified protocols that allows continous performances of cDNA synthesis and PCR reaction in one tube, only requiring addition of the template RNA and its specific primer
- Stability
Addition of stabilizer and RNase inhibitor for high resistance to degradation
- Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables
Application
• First-strand synthesis of cDNA from RNA molecules (RT)
• RT-PCR
• cDNA library construction
• Gene-expression analysis
Experimental data
![rt pcr figure1](/images/products/RT_PCR_PreMix_f01.gif)
Figure. Specific amplification of 5'- UTR region of HCV with AccuPower® RT/PCR PreMix.
Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix. Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.
Lane 1; DNA Ladder (BM corp.)
Lane 2; negative control
Lane 3, 4; HCV positive serum
Lane 5; HCV negative serum