AccuPower® RT MasterMix (2X, 1ml)

AccuPower^® RT Master Mix is the Master Mix type of AccuPower^® RT Premix products.

※ Master Mix product is shipped in dry ice

$104.00

Cat. No.

K-2263

Product Overview

Features and Benefits

Easy-of-use
Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests easily simply by adding template DNA and primers
Stability
Addition of stabilizer and RNase inhibitor for high resistance to degradation
Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables

Application

• First-strand synthesis of cDNA from RNA molecules (RT)

• Random priming reaction

• cDNA library construction

• Probe labeling

• mRNA 5’-end mapping by primer extension analysis

• PCR

• Real-time qPCR

Experimental data

Figure. Amplification of an GAPDH target gene was detected using human total RNA (from 10 ng to 10 pg ) with AccuPower^® RT PreMix.
Lanes 1-4; 10 ng, 1 ng ,and 100 pg, 10 pg of total RNA from HeLa cells, respectively.

Technical/Specs

▶ Specifications

DNase activity	No
RNase activity	No
Fragment size	Up to 9 kb

▶ Experimental data

Figure 1. Specific amplification of 5'- UTR region of HCV with AccuPower^® RT PreMix

lane 1; 1, 2, 3 DNA Ladder (BM corp.)
lane 2; negative control
lane 3, 4; HCV positive serum
lane 5; HCV negative serum

Figure 2. AccuPower^® RT PreMix from each lot was tested to confirm reliability and reproducibility.
Human total RNA (panel A; actin, panel B; globin) and hog cholera virus RNA (panel C) were used as template. Following cDNA synthesis, AccuPower^®PCR PreMix was used to amplify target genes.

Lane M; 100 bp DNA Ladder (Cat. No.: D-1030)
Lane 1-4; Reliability test of each lot with AccuPower^® RT PreMix

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat.No.	Product Description	Option				Price
K-2041	AccuPower^® RT PreMix & Master Mix	PreMix type	0.2 ml thin-wall 8-strip tubes with attached cap	96 tubes	20 µl/rxn	$K-2041
K-2043				96 tubes	50 µl/rxn (with dye)	$K-2043
K-2041-B				480 tubes	20 µl/rxn	$K-2041-B
K-2043-B				480 tubes	50 µl/rxn (with dye)	$K-2043-B
K-2040			0.5 ml thin-wall tubes with attached cap	100 tubes	20 µl/rxn (with dye)	$K-2040
K-2042			0.5 ml thin-wall tubes with attached cap	100 tubes	50 µl/rxn (with dye)	$K-2042
K-2261-1			thin-wall 96-well flat plate		10 µl/rxn	$K-2261-1
K-2261-4			thin-wall 96-well flat plate		20 µl/rxn	$K-2261-4
K-2261-2			thin-wall 96-well full-skirted plate		10 µl/rxn	$K-2261-2
K-2261-5			thin-wall 96-well full-skirted plate		20 µl/rxn	$K-2261-5
K-2261-3			thin-wall 96-well semi-skirted plate		10 µl/rxn	$K-2261-3
K-2261-6			thin-wall 96-well semi-skirted plate		20 µl/rxn	$K-2261-6
K-2082-1			thin-wall 384-well full-skirted plate		5 µl/rxn	$K-2082-1
K-2082-2					10 µl/rxn	$K-2082-2
K-2082-3					20 µl/rxn	$K-2082-3
K-2263		MasterMix type	1 ml of 2X Master mix solution			$K-2263

* The RT series does not contain tracking dyes for electrophoresis, with the exception of some products.