AccuPower® Multiplex PCR PreMix (480 T, 50 μl)

AccuPower^® Multiplex PCR PreMix allows to generate 20 multiplexed amplification products in a single tube. By applying antibody-based HotStart Top DNA Polymerase, it reduces non-specific reactions such as mis-priming and primer-dimer during DNA polymerase reaction at a low temperature. This product is applicable for normal and genotyping assays using multiplex PCR technology, along with semi-quantitative gene expression experiments using cDNA.

※ This product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.

$620.00

Cat. No.

K-2114

Product Overview

Features and Benefits

Multiplex PCR
Generation of 20 multiplexed amplification products in a single tube.
Specificity & Efficiency
Minimized unnecessary reactions such as mis-priming and primer dimer with excellent specificity and amplification efficiency than with other products when compared with same PCR cycles by the use of Hotstart Top DNA polymerase
User-friendly
PCR condition optimization not required with precise specificity of the product only requiring addition of template DNA and primers for anybody to simultaneously amplify the target DNA
Stability
Enhanced stability compared to solution-type products allowing delivery at room temperature by addition of stabilizing agents
Reproducibility
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables through the mass production under ISO 9001 quality system

Application

• STR analysis

• Molecular diagnostic analysis

• Qualitative, semi-qualitative gene expression assay

• Mutant screening

• Transgenic organism analysis

• Genotyping assay

Experimental data

Figure. Comparison of amplification quality between AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit.
10-plex primers were added into AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).

Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl₂).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 65°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer)

Technical/Specs

▶Specifications

Enzyme	HotStart Top DNA polymerase
5' to 3' exonuclease	No
3' to 5' exonuclease	No
3' – A overhang	Yes
Fragment Size	Up to 1 kb

▶ Experimental data

Figure 1. Single PCR and multiplex PCR using AccuPower^® Multiplex PCR PreMix

Each lane from left to right indicates the single and multiplex PCR product using AccuPower^® Multiplex PCR PreMix.
a) 10 plex multiplex PCR, b) 20 plex multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)

Figure 2. Comparison of amplification quality between AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

6-plex primers were added into AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of Human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl₂).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 60°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)

Figure 3. Comparison of amplification quality using Lapchip^® between AccuPower^® Multiplex PCR PreMix and other supplier's master mix.

a) Virtual gel image. Gel image illustrates data reproducibility of the Lapchip^® 90 system.
b) Overlay of expression level using Bioneer's multiplex PCR PreMix and other company's multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower^® Multiplex PCR PreMix and other supplier's master mix.

Figure 4. Comparison of amplification quality between AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

20-plex primers were added into AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (100 ng ~ 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.)
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl₂)
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 57°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer) Trademark: 'AccuPower^®' is a registered trademark of Bioneer Corporation.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat. No.	Product Description	Option			Price
K-2111	AccuPower^® Multiplex PCR PreMix & Master Mix	PreMix type	0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes	20 μl reaction	$K-2111
K-2112			0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes	50 μl reaction	$K-2112
K-2113			0.2 ml thin-wall 8-tube strips with attached cap / 480 tubes	20 μl reaction	$K-2113
K-2114				50 μl reaction	$K-2114
K-2120		MasterMix type	MasterMix type, 1 ml of 2X Master mix solution		$K-2120