Top DNA Polymerase, 10X Reaction Buffer with MgCl2 (2,000 units)

Top DNA polymerase contains a thermostable DNA polymerase having improved polymerase activity by using the recombinant DNA technology on a thermophilic polymerase gene.

※This product is shipped in dry ice.

$249.00

Cat. No.

E-3101-2

Product Overview

Features and Benefits

Optimized buffer delivery
Guaranteed stable response with Top optimized buffer for DNA polymerase
Efficiency & Sensitivity
High efficiency and sensitivity for DNA amplification using PCR
Reproducibility
Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system

Application

Real-time quantification of DNA and cDNA targets using dsDNA binding dye
Gene expression profiling
Microbial & viral pathogen detection

Experimental data

Figure. Sensitivity test of Top DNA polymerase using Lambda genomic DNA.
Each fragment was amplified from a template dilution series (10 ng to 10 fg DNA per reaction) using 1 U of each Top DNA polymerase.

Technical/Specs

▶ Specifications

5' to 3' exonuclease activity	No
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment size	Up to 10 kb

▶ Components

• 10X reaction buffer with (or without) MgCl₂: Tris (pH 9.0), 15 mM MgCl₂, etc

• 1X dilution buffer: 50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0

• 10 mM dNTPs mix: 2.5 mM of each dNTP

▶ Concentration

500 Units (5 U/μl)

▶ Storage conditions

50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0

▶ Storage temperature

-20°C

▶ Unit Definition

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.

▶ Experimental data

Figure 1. Sensitivity test of Top DNA polymerase using Human genomic DNA. Each fragment was amplified from a template dilution series (10 ng to 10 fg DNA per reaction) using 1 U of each Top DNA polymerase.

Figure 2. Enzyme activity test of Top DNA Polymerase. Top DNA polymerase was serially diluted and used to amplify 10 ng of each human genomic DNA.

※ The 5’ -> 3’ exonuclease activity was inhibited in this Top DNA polymerase enzyme through point mutation to enhance the base incorporation rate. Thus, this product can be used with Real-time PCR used with dsDNA binding dyes, but not when it is used with probes. If the latter material must be used, it is highly recommended to use AccuPower®Dualstar™ qPCR PreMix.

Literature/Support

◈ Manual

• Manual_Top DNA polymerase

• Manual_10X Reaction buffer for Top DNA Polymerase

• Manual_10 mM dNTPs

◈ MSDS

• MSDS_10 mM dNTPs

• MSDS_20 mM MgCl₂

• MSDS_Top DNA polymerase

• MSDS_Dilution buffer for Top DNA polymerase

• MSDS_10X_Reaction buffer for Top DNA polymerase

• MSDS_10X Reaction buffer without MgCl2 for Top DNA polymerase

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option			Price
E-3100	Top DNA Polymerase	500 U	10X reaction buffer with MgCl₂	10 mM dNTPs	$E-3100
E-3100-2			10X reaction buffer with MgCl₂		$E-3100-2
E-3100-1			10X reaction buffer, 20 mM MgCl₂	10 mM dNTPs	$E-3100-1
E-3100-3			10X reaction buffer, 20 mM MgCl₂		$E-3100-3
E-3101		2,000 U	10X reaction buffer with MgCl₂	10 mM dNTPs	$E-3101
E-3101-2			10X reaction buffer with MgCl₂		$E-3101-2
E-3101-1			10X reaction buffer, 20 mM MgCl₂	10 mM dNTPs	$E-3101-1
E-3101-3			10X reaction buffer, 20 mM MgCl₂		$E-3101-3
D-3001	10 mM dNTP	1 ml (each 2.5 mM)			$D-3001