Features and Benefits
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Cost-effective kit
No need of additional costly instruments other than the kit and MagListo™ Magnetic Separation Rack
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Comprehensive sample compatibility
A broad range of cell-free DNA extraction from samples such as plasma, serum, urine, saliva, etc.
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Convenience
Wide compatibility for scales of Mini, Midi, or Maxi isolation protocols with just a single kit
Application
• PCR
• Real-time PCR
• Digital PCR
• Next-generation sequencing (NGS)
▶ Specifications
Sample type |
Mini |
Midi |
Maxi |
Plasma / Serum / Urine / Saliva |
~ 1 ml |
~ 3 ml |
~ 5 ml |
Elution volume |
30 μl |
50 μl |
70 μl |
Typical DNA yield |
~5 ng |
~ 15 ng |
~ 25 ng |
* Minor differences in the size may be found in the actual product.
▶ Components
Reagent |
*K-3619 |
CL buffer |
110 ml x 1 ea |
CB buffer |
110 ml x 1 ea |
CW1 buffer |
110 ml x 1 ea |
TA buffer |
120 ml x 1 ea |
EA buffer |
25 ml x 1 ea |
Magnetic Nanobead |
11 ml x 1 ea |
Proteinase K |
25 mg x 2 ea |
*mini - 50 rxn, midi - 16 rxn, maxi - 10 rxn
▶ Experimental data
a) Plasma |
b) Urine |
c) Saliva |
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Figure 1. Size of cfDNA from EDTA human plasma, urine and saliva mixed with Bioneer ladder purified with MagListo™ cfDNA Extraction Kit.
M: Bioneer 25/100bp mixed ladder (Cat. No.D-1020)
a) 1-3: cfDNA of the plasma mixed Bioneer ladder purified with Bioneer MagListo™ Kit
b) 1-3: cfDNA of the urine mixed Bioneer ladder purified with Bioneer MagListo™ Kit
c) 1-3: 1-3: cfDNA of the saliva mixed Bioneer ladder purified with Bioneer MagListo™ Kit
a) Plasma |
b) Serum |
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Figure 2. cfDNA purified with MagListo™ cfDNA Extraction Kit from EDTA human plasma, serum
M: Bioneer 25/100bp mixed ladder (Cat. No.D-1020)
a) cfDNA of the plasma mixed Bioneer ladder purified with Bioneer MagListo™ Kit
b) cfDNA of the serum purified with Bioneer MagListo™ Kit
a) Serum
Figure3. Cell-free DNA extracted from a normal human serum (Millipore, 2 ml) purified with MagListo™ cfDNA Extraction kit and compared with a competitor’s kit (magnetic bead type)
Comparison of cell-free DNA yield purified with MagListo™ cfDNA Extraction ki.
M: Bioneer 25 /100 bp mixed ladder
1-3: Cell-free DNA from the serum purified with Bioneer MagListo™
4-6: Cell-free DNA from the serum purified with competitor T kit