- Verotoxigenic E. coli, (VTEC) is a typical food poisoning-causing pathogenic Escherichia coli. It causes food poisoning such as abdominal pain, fever, nausea, vomiting, hemorrhagic colitis and hemolytic uremic syndrome when food contaminated with it is ingested.
- This test method detects both E. coli O157:H7 and verotoxigenic E. coli.
- Synonym : Shigatoxigenic Escherichia coli (STEC), Enterohemorrhage E. coli (EHEC), Vero-toxin producing E. coli(VTEC)
Features and Benefits
> Symplicity
Tracking dyes and sedimentation are included, so not necessary sample loading buffers when electrophoresis. Therefore, carry-over contamination by aerosol generation can be minimized.
> Excellent reproducibility
Use of dried-type PCR PreMix only requires simple addition of template DNA and DW, which provides highly reproducible results.
> Prevention of amplicon-induced cross-contamination by 8-MOP
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.
> High Stability
Two-year shelf life guaranteed at -20 °C.
> Quality Control
All AccuPower® products are manufactured under ISO 9001 Quality Control System.
* This product is manufactured in accordance with the test method for Enterohemorrhagic Escherichia coli in Korean Food Standards Codex.
Specifications
| Instrument |
AllInOneCycler™ Thermal Block or other Thermal Block |
| Specimen |
Food, Environmental sample etc. |
| Time to result |
~ 2hr. 40min. |
| Tests per Kit |
96 |
Performances
Figure 1. Performance test of AccuPower® VTEC Taq PCR kit(Veterinary) Sensitivity tests were performed by diluting the positive control VT1 DNA and VT2 DNA in 10-fold increments (100 pg to 100 fg for samples 1 to 4). The reaction conditions were 95 ℃ for 60 sec. 65 ℃ 120 sec. 72 ℃ for 90 seconds (10 cycles), 95 ℃ for 60 seconds. 65 ℃ 120 sec. 72 ℃ for 90 seconds (10 cycles), 95 ℃ for 60 seconds. 64 ℃ -> 60 ℃ (1 ℃ / decrease) 120sec. 72 ℃ for 90 seconds (5 cycles), 95 ℃ for 60 seconds. 60 ℃ 120 sec. 72 ℃ for 90 seconds (10 cycles), 95 ℃ for 60 seconds. 60 ℃ 120 sec. The PCR reaction was carried out at 72 ℃ for 150 seconds (10 cycles) and 4 ℃ storage conditions. The 5㎕ PCR reaction product was electrophoresed on 2% TBE agarose gel.
Line M : Marker
Line 1 : Vet_VT1 DNA 500pg + VT2 DNA 500pg
Line 2 : Vet_VT1 DNA 50pg + VT2 DNA 50pg
Line 3 : Vet_VT1 DNA 5pg + VT2 DNA 5pg
Line 4 : Vet_VT1 DNA 500fg + VT2 DNA 500fg
Line N : No template control
