The CRISPR/Cas9 or Cpf1 system uses a genome editing technology to modify genes using Nuclease (Cas9 or Cpf1) and gRNA (guide RNA) complexes.
Bioneer’s AccuCRISPR™-Cas9/Cpf1 gRNA offers customized gene synthesis service. Simply enter the sequences known from your past experiences or a research paper in the order manual.
The ordered gRNA can be used in the form of ribonucleoprotein (RNP) with Nuclease (Cas9 or Cpf1). Since the RNP form can be rapidly degraded by endogenous protease or RNase, It has the advantage of lower off-target.
Bioneer offers CRISPR-gRNA service based on a long-term oligo synthesis technology. All the gRNAs have passed RNA quality control (QC) through synthesis in our state-of-the-art clean room facility and purification using MALDI-TOF mass spectrometry.
Bioneer provides gRNA compatible with two types of Cpf1: AsCpf1 and LbCpf1.
If you enter a target-specific sequence of 20 nt on the order page, a fixed direct repeat sequence is automatically added and ordered. The provided gRNA(crRNA) can be used in the form of RNP (Ribonucleoprotein)
Ready-to-use guide RNAs compatible with nuclease without the need of cloning process
Qualified products through the synthesis in a sterile room
Advanced RNA oligo synthesis technology
Comprehensive gRNA design compatible with various CRISPR nuclease
Figure1. In-vitro digestion of pBHA vector (plasmid & PCR product) using LbCpf1 nuclease and Bioneer’s gRNA. Products were resolved on a 1% agarose TAE gel.
- Plasmid & PCR product size: 2000bp
- cleaved PCR fragment size: 700bp + 1300bp
